Determination of cholesterol and four phytosterols in foods without derivatization by gas chromatography-tandem mass spectrometry

In this study, a method for determination of cholesterol and four phytosterols by gas chromatography coupled with electron impact ionization mode–tandem mass spectrometry without derivatization in general food was developed. The sample was saponified with 7.5% KOH in methanol. After heating on hot p...

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Detalles Bibliográficos
Autores principales: Chen, Yan-Zong, Kao, Shih-Yao, Jian, Hao-Cheng, Yu, Yu-Man, Li, Ju-Ying, Wang, Wei-Hsien, Tsai, Chung-Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Taiwan Food and Drug Administration 2015
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345442/
https://www.ncbi.nlm.nih.gov/pubmed/28911479
http://dx.doi.org/10.1016/j.jfda.2015.01.010
Descripción
Sumario:In this study, a method for determination of cholesterol and four phytosterols by gas chromatography coupled with electron impact ionization mode–tandem mass spectrometry without derivatization in general food was developed. The sample was saponified with 7.5% KOH in methanol. After heating on hot plate and reflux for 60 minutes, the saponified portion was extracted with n-hexane/petroleum ether (50:50, v/v). The extracts were evaporated with rotary evaporator and then redissolved with tetrahydrofuran. The tetra-hydrofuran layer was transferred into an injection vial and analyzed by gas chromatography on a 30 m VF-5 column. Limit of quantification was 2 mg/kg. Recoveries of cholesterol and four phytosterols from general food were between 91% and 100%.

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