Multiplex base editing to convert TAG into TAA codons in the human genome

Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here we take the first steps to extend this to human cells demonstrating exceptional base editing to convert TAG to TAA for 33 essential genes via a single transfection, and exami...

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Autores principales: Chen, Yuting, Hysolli, Eriona, Chen, Anlu, Casper, Stephen, Liu, Songlei, Yang, Kevin, Liu, Chenli, Church, George
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345975/
https://www.ncbi.nlm.nih.gov/pubmed/35918324
http://dx.doi.org/10.1038/s41467-022-31927-8
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author Chen, Yuting
Hysolli, Eriona
Chen, Anlu
Casper, Stephen
Liu, Songlei
Yang, Kevin
Liu, Chenli
Church, George
author_facet Chen, Yuting
Hysolli, Eriona
Chen, Anlu
Casper, Stephen
Liu, Songlei
Yang, Kevin
Liu, Chenli
Church, George
author_sort Chen, Yuting
collection PubMed
description Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here we take the first steps to extend this to human cells demonstrating exceptional base editing to convert TAG to TAA for 33 essential genes via a single transfection, and examine base-editing genome-wide (observing ~40 C-to-T off-target events in essential gene exons). We also introduce GRIT, a computational tool for recoding. This demonstrates the feasibility of recoding, and highly multiplex editing in mammalian cells.
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spelling pubmed-93459752022-08-04 Multiplex base editing to convert TAG into TAA codons in the human genome Chen, Yuting Hysolli, Eriona Chen, Anlu Casper, Stephen Liu, Songlei Yang, Kevin Liu, Chenli Church, George Nat Commun Article Whole-genome recoding has been shown to enable nonstandard amino acids, biocontainment and viral resistance in bacteria. Here we take the first steps to extend this to human cells demonstrating exceptional base editing to convert TAG to TAA for 33 essential genes via a single transfection, and examine base-editing genome-wide (observing ~40 C-to-T off-target events in essential gene exons). We also introduce GRIT, a computational tool for recoding. This demonstrates the feasibility of recoding, and highly multiplex editing in mammalian cells. Nature Publishing Group UK 2022-08-02 /pmc/articles/PMC9345975/ /pubmed/35918324 http://dx.doi.org/10.1038/s41467-022-31927-8 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Chen, Yuting
Hysolli, Eriona
Chen, Anlu
Casper, Stephen
Liu, Songlei
Yang, Kevin
Liu, Chenli
Church, George
Multiplex base editing to convert TAG into TAA codons in the human genome
title Multiplex base editing to convert TAG into TAA codons in the human genome
title_full Multiplex base editing to convert TAG into TAA codons in the human genome
title_fullStr Multiplex base editing to convert TAG into TAA codons in the human genome
title_full_unstemmed Multiplex base editing to convert TAG into TAA codons in the human genome
title_short Multiplex base editing to convert TAG into TAA codons in the human genome
title_sort multiplex base editing to convert tag into taa codons in the human genome
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9345975/
https://www.ncbi.nlm.nih.gov/pubmed/35918324
http://dx.doi.org/10.1038/s41467-022-31927-8
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