Improved ClickTags enable live-cell barcoding for highly multiplexed single cell sequencing

Click chemistry-enabled DNA barcoding of cells provides a universal strategy for sample multiplexing in single-cell RNA-seq (scRNA-seq). However, current ClickTags are limited to fixed samples as they only label cells efficiently in methanol. Herein, we report the development of a new protocol for b...

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Detalles Bibliográficos
Autores principales: Zhao, Xinlu, Sun, Shiming, Yu, Wenhao, Zhu, Wenqi, Zhao, Zihan, Zhou, Yiqi, Ding, Xiuheng, Fang, Nan, Yang, Rong, Li, Jie P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: RSC 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347365/
https://www.ncbi.nlm.nih.gov/pubmed/35975006
http://dx.doi.org/10.1039/d2cb00046f

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9347365/
https://www.ncbi.nlm.nih.gov/pubmed/35975006
http://dx.doi.org/10.1039/d2cb00046f

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