Implementation of a Design of Experiments to Improve Periplasmic Yield of Functional ScFv Antibodies in a Phage Display Platform

Purpose: Production of functional recombinant antibody fragments in the periplasm of E. coli is a prerequisite step to achieve sufficient reagent for preclinical studies. Thus, the cost-effective and lab-scale production of antibody fragments demands the optimization of culture conditions. Methods:...

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Detalles Bibliográficos
Autores principales: Aghdam, Marjan Abri, Tohidkia, Mohammad Reza, Ghamghami, Elham, Ahmadikhah, Asadollah, Khanmahamadi, Morteza, Baradaran, Behzad, Mokhtarzadeh, Ahad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tabriz University of Medical Sciences 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348535/
https://www.ncbi.nlm.nih.gov/pubmed/35935041
http://dx.doi.org/10.34172/apb.2022.061

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9348535/
https://www.ncbi.nlm.nih.gov/pubmed/35935041
http://dx.doi.org/10.34172/apb.2022.061

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