Variations on a theme: crystal forms of the amino-acid transporter MhsT

The bacterial amino-acid transporter MhsT from the SLC6A family has been crystallized in complex with different substrates in order to understand the determinants of the substrate specificity of the transporter. Surprisingly, crystals of the different MhsT–substrate complexes showed interrelated but...

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Detalles Bibliográficos
Autores principales: Neumann, Caroline, Focht, Dorota, Trampari, Sofia, Lyons, Joseph A., Nissen, Poul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Research Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350835/
https://www.ncbi.nlm.nih.gov/pubmed/35924597
http://dx.doi.org/10.1107/S2053230X22007154
Descripción
Sumario:The bacterial amino-acid transporter MhsT from the SLC6A family has been crystallized in complex with different substrates in order to understand the determinants of the substrate specificity of the transporter. Surprisingly, crystals of the different MhsT–substrate complexes showed interrelated but different crystal-packing arrangements. Space-group assignment and structure determination of these different crystal forms present challenging combinations of pseudosymmetry, twinning and translational non­crystallographic symmetry.

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