Variations on a theme: crystal forms of the amino-acid transporter MhsT

The bacterial amino-acid transporter MhsT from the SLC6A family has been crystallized in complex with different substrates in order to understand the determinants of the substrate specificity of the transporter. Surprisingly, crystals of the different MhsT–substrate complexes showed interrelated but...

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Autores principales: Neumann, Caroline, Focht, Dorota, Trampari, Sofia, Lyons, Joseph A., Nissen, Poul
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2022
Materias:
Research Communications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350835/
https://www.ncbi.nlm.nih.gov/pubmed/35924597
http://dx.doi.org/10.1107/S2053230X22007154
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author Neumann, Caroline
Focht, Dorota
Trampari, Sofia
Lyons, Joseph A.
Nissen, Poul
author_facet Neumann, Caroline
Focht, Dorota
Trampari, Sofia
Lyons, Joseph A.
Nissen, Poul
author_sort Neumann, Caroline
collection PubMed
description The bacterial amino-acid transporter MhsT from the SLC6A family has been crystallized in complex with different substrates in order to understand the determinants of the substrate specificity of the transporter. Surprisingly, crystals of the different MhsT–substrate complexes showed interrelated but different crystal-packing arrangements. Space-group assignment and structure determination of these different crystal forms present challenging combinations of pseudosymmetry, twinning and translational non­crystallographic symmetry.
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spelling pubmed-93508352022-08-15 Variations on a theme: crystal forms of the amino-acid transporter MhsT Neumann, Caroline Focht, Dorota Trampari, Sofia Lyons, Joseph A. Nissen, Poul Acta Crystallogr F Struct Biol Commun Research Communications The bacterial amino-acid transporter MhsT from the SLC6A family has been crystallized in complex with different substrates in order to understand the determinants of the substrate specificity of the transporter. Surprisingly, crystals of the different MhsT–substrate complexes showed interrelated but different crystal-packing arrangements. Space-group assignment and structure determination of these different crystal forms present challenging combinations of pseudosymmetry, twinning and translational non­crystallographic symmetry. International Union of Crystallography 2022-07-26 /pmc/articles/PMC9350835/ /pubmed/35924597 http://dx.doi.org/10.1107/S2053230X22007154 Text en © Caroline Neumann et al. 2022 https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.
spellingShingle Research Communications
Neumann, Caroline
Focht, Dorota
Trampari, Sofia
Lyons, Joseph A.
Nissen, Poul
Variations on a theme: crystal forms of the amino-acid transporter MhsT
title Variations on a theme: crystal forms of the amino-acid transporter MhsT
title_full Variations on a theme: crystal forms of the amino-acid transporter MhsT
title_fullStr Variations on a theme: crystal forms of the amino-acid transporter MhsT
title_full_unstemmed Variations on a theme: crystal forms of the amino-acid transporter MhsT
title_short Variations on a theme: crystal forms of the amino-acid transporter MhsT
title_sort variations on a theme: crystal forms of the amino-acid transporter mhst
topic Research Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350835/
https://www.ncbi.nlm.nih.gov/pubmed/35924597
http://dx.doi.org/10.1107/S2053230X22007154
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