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Synthesis of Novel Phthalazinedione-Based Derivatives with Promising Cytotoxic, Anti-bacterial, and Molecular Docking Studies as VEGFR2 Inhibitors
[Image: see text] The parent ester methyl-3-[2-(4-oxo-3-phenyl-3,4-dihydro-phthalazin-1-yloxy)-acetylamino] has 18 compounds. The starting material for alkanoates, their corresponding hydrazides, hydrazones, and dipeptides were produced by chemoselective O-alkylation of 2-phenyl-2,3-dihydrophthalazi...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350887/ https://www.ncbi.nlm.nih.gov/pubmed/35936456 http://dx.doi.org/10.1021/acsomega.2c03182 |
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author | El Rayes, Samir M. El Enany, Gaber Ali, Ibrahim A. I. Ibrahim, Wessam Nafie, Mohamed S. |
author_facet | El Rayes, Samir M. El Enany, Gaber Ali, Ibrahim A. I. Ibrahim, Wessam Nafie, Mohamed S. |
author_sort | El Rayes, Samir M. |
collection | PubMed |
description | [Image: see text] The parent ester methyl-3-[2-(4-oxo-3-phenyl-3,4-dihydro-phthalazin-1-yloxy)-acetylamino] has 18 compounds. The starting material for alkanoates, their corresponding hydrazides, hydrazones, and dipeptides were produced by chemoselective O-alkylation of 2-phenyl-2,3-dihydrophthalazine-1,4-dione with ethyl chloroacetate(4-oxo-3-phenyl-3,4-dihydro-phthalazin-1-yloxy) acetic acid methyl ester. The starting ester was hydrazinolyzed, then azide coupled with amino acid ester hydrochloride to produce several parent esters, and then hydrazinolyzed to produce parent hydrazides. These hydrazides were used to make a series of dipeptides by reacting them with amino acid ester hydrochloride under azide coupling conditions, and they were also condensed with a number of aldehydes to make the hydrazones. These derivatives were subjected to cytotoxicity against HCT-116 and MDA-MB-231 cells and anti-bacterial and molecular docking studies. Results indicated that the tested compounds, especially 7c and 8b with the phenyl phthalazinone moieties, had promising cytotoxicity against the HCT-116 cells with IC(50) values of 1.36 and 2.34 μM, respectively. Additionally, the promising compounds 7c and 8b exhibited poor cytotoxicity against WISH cells with much higher IC(50) values, so they were safe against normal cells. Compound 8c exhibited potent anti-bacterial activity with inhibition zones of 12 and 11 mm against Staphylococcus aureus and Escherichia coli, respectively. The molecular docking results of compounds 7c and 8b revealed a good binding disposition and the ligand–receptor interactions like the co-crystallized ligand of the VEGFR2 protein, which may be the proposed mode of action. Finally, compounds 7c and 8b exhibited good ADME pharmacokinetics with good drug-likeness parameters. Hence, detailed studies for the mechanism of action of such compounds are highly recommended for the development of new potent anti-cancer and anti-bacterial agents. |
format | Online Article Text |
id | pubmed-9350887 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-93508872022-08-05 Synthesis of Novel Phthalazinedione-Based Derivatives with Promising Cytotoxic, Anti-bacterial, and Molecular Docking Studies as VEGFR2 Inhibitors El Rayes, Samir M. El Enany, Gaber Ali, Ibrahim A. I. Ibrahim, Wessam Nafie, Mohamed S. ACS Omega [Image: see text] The parent ester methyl-3-[2-(4-oxo-3-phenyl-3,4-dihydro-phthalazin-1-yloxy)-acetylamino] has 18 compounds. The starting material for alkanoates, their corresponding hydrazides, hydrazones, and dipeptides were produced by chemoselective O-alkylation of 2-phenyl-2,3-dihydrophthalazine-1,4-dione with ethyl chloroacetate(4-oxo-3-phenyl-3,4-dihydro-phthalazin-1-yloxy) acetic acid methyl ester. The starting ester was hydrazinolyzed, then azide coupled with amino acid ester hydrochloride to produce several parent esters, and then hydrazinolyzed to produce parent hydrazides. These hydrazides were used to make a series of dipeptides by reacting them with amino acid ester hydrochloride under azide coupling conditions, and they were also condensed with a number of aldehydes to make the hydrazones. These derivatives were subjected to cytotoxicity against HCT-116 and MDA-MB-231 cells and anti-bacterial and molecular docking studies. Results indicated that the tested compounds, especially 7c and 8b with the phenyl phthalazinone moieties, had promising cytotoxicity against the HCT-116 cells with IC(50) values of 1.36 and 2.34 μM, respectively. Additionally, the promising compounds 7c and 8b exhibited poor cytotoxicity against WISH cells with much higher IC(50) values, so they were safe against normal cells. Compound 8c exhibited potent anti-bacterial activity with inhibition zones of 12 and 11 mm against Staphylococcus aureus and Escherichia coli, respectively. The molecular docking results of compounds 7c and 8b revealed a good binding disposition and the ligand–receptor interactions like the co-crystallized ligand of the VEGFR2 protein, which may be the proposed mode of action. Finally, compounds 7c and 8b exhibited good ADME pharmacokinetics with good drug-likeness parameters. Hence, detailed studies for the mechanism of action of such compounds are highly recommended for the development of new potent anti-cancer and anti-bacterial agents. American Chemical Society 2022-06-27 /pmc/articles/PMC9350887/ /pubmed/35936456 http://dx.doi.org/10.1021/acsomega.2c03182 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | El Rayes, Samir M. El Enany, Gaber Ali, Ibrahim A. I. Ibrahim, Wessam Nafie, Mohamed S. Synthesis of Novel Phthalazinedione-Based Derivatives with Promising Cytotoxic, Anti-bacterial, and Molecular Docking Studies as VEGFR2 Inhibitors |
title | Synthesis of Novel
Phthalazinedione-Based Derivatives
with Promising Cytotoxic, Anti-bacterial, and Molecular Docking Studies
as VEGFR2 Inhibitors |
title_full | Synthesis of Novel
Phthalazinedione-Based Derivatives
with Promising Cytotoxic, Anti-bacterial, and Molecular Docking Studies
as VEGFR2 Inhibitors |
title_fullStr | Synthesis of Novel
Phthalazinedione-Based Derivatives
with Promising Cytotoxic, Anti-bacterial, and Molecular Docking Studies
as VEGFR2 Inhibitors |
title_full_unstemmed | Synthesis of Novel
Phthalazinedione-Based Derivatives
with Promising Cytotoxic, Anti-bacterial, and Molecular Docking Studies
as VEGFR2 Inhibitors |
title_short | Synthesis of Novel
Phthalazinedione-Based Derivatives
with Promising Cytotoxic, Anti-bacterial, and Molecular Docking Studies
as VEGFR2 Inhibitors |
title_sort | synthesis of novel
phthalazinedione-based derivatives
with promising cytotoxic, anti-bacterial, and molecular docking studies
as vegfr2 inhibitors |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9350887/ https://www.ncbi.nlm.nih.gov/pubmed/35936456 http://dx.doi.org/10.1021/acsomega.2c03182 |
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