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Parameter estimation on multivalent ITC data sets

The Wiseman fitting can be used to extract binding parameters from ITC data sets, such as heat of binding, number of binding sites, and the overall dissociation rate. The classical Wiseman fitting assumes a direct binding process and neglects the possibility of intermediate binding steps. In princip...

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Detalles Bibliográficos
Autores principales: Erlekam, Franziska, Zumbansen, Maximilian, Weber, Marcus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9352788/
https://www.ncbi.nlm.nih.gov/pubmed/35927455
http://dx.doi.org/10.1038/s41598-022-17188-x