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Intramuscular Inoculation of AS02-Adjuvanted Respiratory Syncytial Virus (RSV) F Subunit Vaccine Shows Better Efficiency and Safety Than Subcutaneous Inoculation in BALB/c Mice

We previously explored a panel of adjuvants formulated with pre-fusion RSV-F protein and found that AS02 may be a promising candidate adjuvant for developing RSV-F subunit vaccines with improved immunogenicity and desired immune response type. In this study, we performed a head-to-head comparison of...

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Autores principales: Bian, Lijun, Zheng, Yu, Guo, Xiaohong, Li, Dongdong, Zhou, Jingying, Jing, Linyao, Chen, Yan, Lu, Jingcai, Zhang, Ke, Jiang, Chunlai, Zhang, Yong, Kong, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354885/
https://www.ncbi.nlm.nih.gov/pubmed/35935960
http://dx.doi.org/10.3389/fimmu.2022.938598
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author Bian, Lijun
Zheng, Yu
Guo, Xiaohong
Li, Dongdong
Zhou, Jingying
Jing, Linyao
Chen, Yan
Lu, Jingcai
Zhang, Ke
Jiang, Chunlai
Zhang, Yong
Kong, Wei
author_facet Bian, Lijun
Zheng, Yu
Guo, Xiaohong
Li, Dongdong
Zhou, Jingying
Jing, Linyao
Chen, Yan
Lu, Jingcai
Zhang, Ke
Jiang, Chunlai
Zhang, Yong
Kong, Wei
author_sort Bian, Lijun
collection PubMed
description We previously explored a panel of adjuvants formulated with pre-fusion RSV-F protein and found that AS02 may be a promising candidate adjuvant for developing RSV-F subunit vaccines with improved immunogenicity and desired immune response type. In this study, we performed a head-to-head comparison of the effect of intramuscular injection to that of subcutaneous injection on the immune response and protective efficacy of recombinant RSV-F subunit vaccine with or without adjuvants (Alhydrogel, squalene-based emulsion adjuvants MF59, AS03, and AS02) in BALB/c mice. After inoculations, antigen-specific antibodies, neutralizing antibodies, antibody subtypes, cytokines, and the persistence of immune response were evaluated. Moreover, challenge tests were also performed to illustrate the possible effect of inoculation routes and adjuvant on virus clearance and histochemistry changes in the lungs of mice. The results indicated that intramuscular inoculation is a more effective and antigen dose-sparing route to enhance the immune response, although subcutaneous inoculation induced faster and stronger IgG antibodies after the initial immunization. Furthermore, adjuvant, but not immunization route, is a more critical factor to affect the humoral/cellular immune response and the immune bias. In addition, adjuvant inoculated via the intramuscular route is safer than that via the subcutaneous route, especially for AS02. This study highlights the importance of the adjuvant and immunization routes in the design and clinical transformation of adjuvanted vaccines. Further investigation is needed to illustrate the mechanism underlying the above difference in both efficiency and safety.
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spelling pubmed-93548852022-08-06 Intramuscular Inoculation of AS02-Adjuvanted Respiratory Syncytial Virus (RSV) F Subunit Vaccine Shows Better Efficiency and Safety Than Subcutaneous Inoculation in BALB/c Mice Bian, Lijun Zheng, Yu Guo, Xiaohong Li, Dongdong Zhou, Jingying Jing, Linyao Chen, Yan Lu, Jingcai Zhang, Ke Jiang, Chunlai Zhang, Yong Kong, Wei Front Immunol Immunology We previously explored a panel of adjuvants formulated with pre-fusion RSV-F protein and found that AS02 may be a promising candidate adjuvant for developing RSV-F subunit vaccines with improved immunogenicity and desired immune response type. In this study, we performed a head-to-head comparison of the effect of intramuscular injection to that of subcutaneous injection on the immune response and protective efficacy of recombinant RSV-F subunit vaccine with or without adjuvants (Alhydrogel, squalene-based emulsion adjuvants MF59, AS03, and AS02) in BALB/c mice. After inoculations, antigen-specific antibodies, neutralizing antibodies, antibody subtypes, cytokines, and the persistence of immune response were evaluated. Moreover, challenge tests were also performed to illustrate the possible effect of inoculation routes and adjuvant on virus clearance and histochemistry changes in the lungs of mice. The results indicated that intramuscular inoculation is a more effective and antigen dose-sparing route to enhance the immune response, although subcutaneous inoculation induced faster and stronger IgG antibodies after the initial immunization. Furthermore, adjuvant, but not immunization route, is a more critical factor to affect the humoral/cellular immune response and the immune bias. In addition, adjuvant inoculated via the intramuscular route is safer than that via the subcutaneous route, especially for AS02. This study highlights the importance of the adjuvant and immunization routes in the design and clinical transformation of adjuvanted vaccines. Further investigation is needed to illustrate the mechanism underlying the above difference in both efficiency and safety. Frontiers Media S.A. 2022-07-22 /pmc/articles/PMC9354885/ /pubmed/35935960 http://dx.doi.org/10.3389/fimmu.2022.938598 Text en Copyright © 2022 Bian, Zheng, Guo, Li, Zhou, Jing, Chen, Lu, Zhang, Jiang, Zhang and Kong https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Bian, Lijun
Zheng, Yu
Guo, Xiaohong
Li, Dongdong
Zhou, Jingying
Jing, Linyao
Chen, Yan
Lu, Jingcai
Zhang, Ke
Jiang, Chunlai
Zhang, Yong
Kong, Wei
Intramuscular Inoculation of AS02-Adjuvanted Respiratory Syncytial Virus (RSV) F Subunit Vaccine Shows Better Efficiency and Safety Than Subcutaneous Inoculation in BALB/c Mice
title Intramuscular Inoculation of AS02-Adjuvanted Respiratory Syncytial Virus (RSV) F Subunit Vaccine Shows Better Efficiency and Safety Than Subcutaneous Inoculation in BALB/c Mice
title_full Intramuscular Inoculation of AS02-Adjuvanted Respiratory Syncytial Virus (RSV) F Subunit Vaccine Shows Better Efficiency and Safety Than Subcutaneous Inoculation in BALB/c Mice
title_fullStr Intramuscular Inoculation of AS02-Adjuvanted Respiratory Syncytial Virus (RSV) F Subunit Vaccine Shows Better Efficiency and Safety Than Subcutaneous Inoculation in BALB/c Mice
title_full_unstemmed Intramuscular Inoculation of AS02-Adjuvanted Respiratory Syncytial Virus (RSV) F Subunit Vaccine Shows Better Efficiency and Safety Than Subcutaneous Inoculation in BALB/c Mice
title_short Intramuscular Inoculation of AS02-Adjuvanted Respiratory Syncytial Virus (RSV) F Subunit Vaccine Shows Better Efficiency and Safety Than Subcutaneous Inoculation in BALB/c Mice
title_sort intramuscular inoculation of as02-adjuvanted respiratory syncytial virus (rsv) f subunit vaccine shows better efficiency and safety than subcutaneous inoculation in balb/c mice
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9354885/
https://www.ncbi.nlm.nih.gov/pubmed/35935960
http://dx.doi.org/10.3389/fimmu.2022.938598
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