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Improving of pelB-Secreted MPT64 protein released by Escherichia coli BL21 (DE3) using Triton X-100 and Tween-80

pelB has been known as a successful signal peptide to translocate the protein target extracellularly in the Escherichia coli system. However, in our previous study, the yield of MPT64 protein extracellular recovery was still low and plenty of this protein was remain trapped in cytoplasm and periplas...

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Detalles Bibliográficos
Autores principales: Kusuma, Sri Agung Fitri, Subroto, Toto, Parwati, Ida, Rukayadi, Yaya, Fadhlillah, Muhammad, Pardede, Ruth Michellee, Berlian, Alif Virisy, Sabila, Gina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355052/
https://www.ncbi.nlm.nih.gov/pubmed/35935695
http://dx.doi.org/10.4103/japtr.japtr_25_22