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Protocol for generation of human peptide-specific primary CD8(+) T cell lines
Directly ex vivo, peptide-specific CD8(+) T cells are present at relatively low frequency and are typically in a resting state. This protocol details the expansion of memory peptide-specific CD8(+) T cells by in vitro stimulation, which can be subsequently characterized using a range of assays inclu...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356162/ https://www.ncbi.nlm.nih.gov/pubmed/35942343 http://dx.doi.org/10.1016/j.xpro.2022.101590 |