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Protocol for generation of human peptide-specific primary CD8(+) T cell lines

Directly ex vivo, peptide-specific CD8(+) T cells are present at relatively low frequency and are typically in a resting state. This protocol details the expansion of memory peptide-specific CD8(+) T cells by in vitro stimulation, which can be subsequently characterized using a range of assays inclu...

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Detalles Bibliográficos
Autores principales: Grant, Emma J., Gras, Stephanie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356162/
https://www.ncbi.nlm.nih.gov/pubmed/35942343
http://dx.doi.org/10.1016/j.xpro.2022.101590