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Experimental and computational workflow for the analysis of tRNA pools from eukaryotic cells by mim-tRNAseq

Quantifying tRNAs is crucial for understanding how they regulate mRNA translation but is hampered by their extensive sequence similarity and premature termination of reverse transcription at multiple modified nucleotides. Here, we describe the use of modification-induced misincorporation tRNA sequen...

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Detalles Bibliográficos
Autores principales: Behrens, Andrew, Nedialkova, Danny D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356165/
https://www.ncbi.nlm.nih.gov/pubmed/35942339
http://dx.doi.org/10.1016/j.xpro.2022.101579