TOP1 CAD-seq: A protocol to map catalytically engaged topoisomerase 1 in human cells

TOP1 CAD-seq enables mapping of TOP1 sites of covalent engagement with DNA. The procedure depends upon enrichment of DNA-covalent adducts using chaotropic salts and immunoprecipitation with an antibody specific for TOP1. Here, we describe a step-by-step protocol compatible with Illumina sequencing a...

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Detalles Bibliográficos
Autores principales: Kuzin, Vladislav, Wiegard, Anika, Cameron, Donald P., Baranello, Laura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356208/
https://www.ncbi.nlm.nih.gov/pubmed/35942340
http://dx.doi.org/10.1016/j.xpro.2022.101581

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356208/
https://www.ncbi.nlm.nih.gov/pubmed/35942340
http://dx.doi.org/10.1016/j.xpro.2022.101581

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