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TOP1 CAD-seq: A protocol to map catalytically engaged topoisomerase 1 in human cells

TOP1 CAD-seq enables mapping of TOP1 sites of covalent engagement with DNA. The procedure depends upon enrichment of DNA-covalent adducts using chaotropic salts and immunoprecipitation with an antibody specific for TOP1. Here, we describe a step-by-step protocol compatible with Illumina sequencing a...

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Detalles Bibliográficos
Autores principales:	Kuzin, Vladislav, Wiegard, Anika, Cameron, Donald P., Baranello, Laura
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9356208/ https://www.ncbi.nlm.nih.gov/pubmed/35942340 http://dx.doi.org/10.1016/j.xpro.2022.101581

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