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OverFlap PCR: A reliable approach for generating plasmid DNA libraries containing random sequences without a template bias
Over the decades, practical biotechnology researchers have aimed to improve naturally occurring proteins and create novel ones. It is widely recognized that coupling protein sequence randomization with various effect screening methodologies is one of the most powerful techniques for quickly, efficie...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359533/ https://www.ncbi.nlm.nih.gov/pubmed/35939421 http://dx.doi.org/10.1371/journal.pone.0262968 |