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OverFlap PCR: A reliable approach for generating plasmid DNA libraries containing random sequences without a template bias

Over the decades, practical biotechnology researchers have aimed to improve naturally occurring proteins and create novel ones. It is widely recognized that coupling protein sequence randomization with various effect screening methodologies is one of the most powerful techniques for quickly, efficie...

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Detalles Bibliográficos
Autores principales:	Linars, Artis, Silamikelis, Ivars, Gudra, Dita, Roga, Ance, Fridmanis, Davids
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9359533/ https://www.ncbi.nlm.nih.gov/pubmed/35939421 http://dx.doi.org/10.1371/journal.pone.0262968

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