The trans DNA cleavage activity of Cas12a provides no detectable immunity against plasmid or phage

Cas12a is a type V-A CRISPR-Cas RNA-guided endonuclease. It cleaves dsDNA at specific site, and then is activated for nonspecific ssDNA cleavage in trans in vitro. The immune function of the trans activity is still unknown. To address this question, we constructed a Cas12a targeting system in Escher...

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Detalles Bibliográficos
Autores principales: Liu, Shunhang, Rao, Xichen, Zhao, Ruiliang, Han, Wenyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Genome Editing
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360544/
https://www.ncbi.nlm.nih.gov/pubmed/35958049
http://dx.doi.org/10.3389/fgeed.2022.929929

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9360544/
https://www.ncbi.nlm.nih.gov/pubmed/35958049
http://dx.doi.org/10.3389/fgeed.2022.929929

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