High-content screening of mitochondrial polarization in neural cells derived from human pluripotent stem cells

We present a high-content screening (HCS) protocol for quantifying mitochondrial activity in live neural cells from human induced pluripotent stem cells (iPSCs). The assessment is based on mitochondrial membrane potential, which is influenced by the efficiency of mitochondrial bioenergetics. We desc...

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Detalles Bibliográficos
Autores principales: Zink, Annika, Haferkamp, Undine, Wittich, Annika, Beller, Mathias, Pless, Ole, Prigione, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9361325/
https://www.ncbi.nlm.nih.gov/pubmed/35959496
http://dx.doi.org/10.1016/j.xpro.2022.101602
Descripción
Sumario:We present a high-content screening (HCS) protocol for quantifying mitochondrial activity in live neural cells from human induced pluripotent stem cells (iPSCs). The assessment is based on mitochondrial membrane potential, which is influenced by the efficiency of mitochondrial bioenergetics. We describe how to perform the analysis using both an HCS platform and the open-source software CellProfiler. The protocol can identify the mitochondrial fitness of human neurons and may be used to carry out high-throughput compound screenings in patient-derived neural cells. For complete details on the use and execution of this protocol, please refer to Lorenz et al. (2017) and Zink et al. (2020).

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