Development of a Handheld Nano-centrifugal Device for Visual Virus Detection
Gold nanoparticles (AuNPs) colorimetric assays based on distance-dependent optical characteristics have been widely employed for bioanalysis. However, this assay is not effective for visually detecting low-concentration targets due to the faint color change. Here, we developed a handheld nano-centri...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Nature Singapore
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9361950/ https://www.ncbi.nlm.nih.gov/pubmed/35966388 http://dx.doi.org/10.1007/s41664-022-00232-0 |
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author | Bi, Zi-Rong Hu, Meng-Lu Jiang, Yong-Zhong Xiong, Er-Hu Shu, Bo-Wen Li, Si-Qi Chen, Han-Wei Chen, Xiao-Hua Zhou, Xiao-Ming |
author_facet | Bi, Zi-Rong Hu, Meng-Lu Jiang, Yong-Zhong Xiong, Er-Hu Shu, Bo-Wen Li, Si-Qi Chen, Han-Wei Chen, Xiao-Hua Zhou, Xiao-Ming |
author_sort | Bi, Zi-Rong |
collection | PubMed |
description | Gold nanoparticles (AuNPs) colorimetric assays based on distance-dependent optical characteristics have been widely employed for bioanalysis. However, this assay is not effective for visually detecting low-concentration targets due to the faint color change. Here, we developed a handheld nano-centrifugal device which could separate the crosslinked and non-crosslinked AuNPs. Results showed that the handheld nano-centrifugal device could easily reach more than 6000 r/min within 10 s simply by stretching and tightening the coiled rope in an appropriate rhythm. Further, combined with the CRISPR/Cas12a nucleic acids recognition system, a field-deployable colorimetric platform termed handheld nano-centrifugal device assisted CRISPR/Cas12a (Hand-CRISPR) has been validated. Moreover, clinical diagnostics applications for Epstein-Barr virus (EBV) and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) detection with high sensitivity and accuracy (100% consistency with reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) test results) have been demonstrated. Overall, the Hand-CRISPR platform showed great promise in point-of-care-test (POCT) application, expected to become a powerful supplement to the standard nucleic acid testing method in remote or poverty-stricken areas. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s41664-022-00232-0. |
format | Online Article Text |
id | pubmed-9361950 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Nature Singapore |
record_format | MEDLINE/PubMed |
spelling | pubmed-93619502022-08-10 Development of a Handheld Nano-centrifugal Device for Visual Virus Detection Bi, Zi-Rong Hu, Meng-Lu Jiang, Yong-Zhong Xiong, Er-Hu Shu, Bo-Wen Li, Si-Qi Chen, Han-Wei Chen, Xiao-Hua Zhou, Xiao-Ming J Anal Test Original Paper Gold nanoparticles (AuNPs) colorimetric assays based on distance-dependent optical characteristics have been widely employed for bioanalysis. However, this assay is not effective for visually detecting low-concentration targets due to the faint color change. Here, we developed a handheld nano-centrifugal device which could separate the crosslinked and non-crosslinked AuNPs. Results showed that the handheld nano-centrifugal device could easily reach more than 6000 r/min within 10 s simply by stretching and tightening the coiled rope in an appropriate rhythm. Further, combined with the CRISPR/Cas12a nucleic acids recognition system, a field-deployable colorimetric platform termed handheld nano-centrifugal device assisted CRISPR/Cas12a (Hand-CRISPR) has been validated. Moreover, clinical diagnostics applications for Epstein-Barr virus (EBV) and severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) detection with high sensitivity and accuracy (100% consistency with reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) test results) have been demonstrated. Overall, the Hand-CRISPR platform showed great promise in point-of-care-test (POCT) application, expected to become a powerful supplement to the standard nucleic acid testing method in remote or poverty-stricken areas. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s41664-022-00232-0. Springer Nature Singapore 2022-08-05 2022 /pmc/articles/PMC9361950/ /pubmed/35966388 http://dx.doi.org/10.1007/s41664-022-00232-0 Text en © The Nonferrous Metals Society of China 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic. |
spellingShingle | Original Paper Bi, Zi-Rong Hu, Meng-Lu Jiang, Yong-Zhong Xiong, Er-Hu Shu, Bo-Wen Li, Si-Qi Chen, Han-Wei Chen, Xiao-Hua Zhou, Xiao-Ming Development of a Handheld Nano-centrifugal Device for Visual Virus Detection |
title | Development of a Handheld Nano-centrifugal Device for Visual Virus Detection |
title_full | Development of a Handheld Nano-centrifugal Device for Visual Virus Detection |
title_fullStr | Development of a Handheld Nano-centrifugal Device for Visual Virus Detection |
title_full_unstemmed | Development of a Handheld Nano-centrifugal Device for Visual Virus Detection |
title_short | Development of a Handheld Nano-centrifugal Device for Visual Virus Detection |
title_sort | development of a handheld nano-centrifugal device for visual virus detection |
topic | Original Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9361950/ https://www.ncbi.nlm.nih.gov/pubmed/35966388 http://dx.doi.org/10.1007/s41664-022-00232-0 |
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