High-throughput proteomic sample preparation using pressure cycling technology

High-throughput lysis and proteolytic digestion of biopsy-level tissue specimens is a major bottleneck for clinical proteomics. Here we describe a detailed protocol of pressure cycling technology (PCT)-assisted sample preparation for proteomic analysis of biopsy tissues. A piece of fresh frozen or f...

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Detalles Bibliográficos
Autores principales: Cai, Xue, Xue, Zhangzhi, Wu, Chunlong, Sun, Rui, Qian, Liujia, Yue, Liang, Ge, Weigang, Yi, Xiao, Liu, Wei, Chen, Chen, Gao, Huanhuan, Yu, Jing, Xu, Luang, Zhu, Yi, Guo, Tiannan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362583/
https://www.ncbi.nlm.nih.gov/pubmed/35931778
http://dx.doi.org/10.1038/s41596-022-00727-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9362583/
https://www.ncbi.nlm.nih.gov/pubmed/35931778
http://dx.doi.org/10.1038/s41596-022-00727-1

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