Expanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides

The oxime ligation is a valuable bioorthogonal conjugation reaction but with limited compatibility with disulfide-rich peptides/proteins and time-sensitive applications. Here we overcome these limitations by introducing a strategy that supports regiospecific control, oxidative folding, production of...

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Detalles Bibliográficos
Autores principales: Hering, Anke, Braga Emidio, Nayara, Muttenthaler, Markus
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367247/
https://www.ncbi.nlm.nih.gov/pubmed/35880482
http://dx.doi.org/10.1039/d2cc03752a
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author Hering, Anke
Braga Emidio, Nayara
Muttenthaler, Markus
author_facet Hering, Anke
Braga Emidio, Nayara
Muttenthaler, Markus
author_sort Hering, Anke
collection PubMed
description The oxime ligation is a valuable bioorthogonal conjugation reaction but with limited compatibility with disulfide-rich peptides/proteins and time-sensitive applications. Here we overcome these limitations by introducing a strategy that supports regiospecific control, oxidative folding, production of stable aminooxy-precursors for on-demand modification, and complete ligation within 5 min.
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spelling pubmed-93672472022-09-08 Expanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides Hering, Anke Braga Emidio, Nayara Muttenthaler, Markus Chem Commun (Camb) Chemistry The oxime ligation is a valuable bioorthogonal conjugation reaction but with limited compatibility with disulfide-rich peptides/proteins and time-sensitive applications. Here we overcome these limitations by introducing a strategy that supports regiospecific control, oxidative folding, production of stable aminooxy-precursors for on-demand modification, and complete ligation within 5 min. The Royal Society of Chemistry 2022-07-26 /pmc/articles/PMC9367247/ /pubmed/35880482 http://dx.doi.org/10.1039/d2cc03752a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Hering, Anke
Braga Emidio, Nayara
Muttenthaler, Markus
Expanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides
title Expanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides
title_full Expanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides
title_fullStr Expanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides
title_full_unstemmed Expanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides
title_short Expanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides
title_sort expanding the versatility and scope of the oxime ligation: rapid bioconjugation to disulfide-rich peptides
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367247/
https://www.ncbi.nlm.nih.gov/pubmed/35880482
http://dx.doi.org/10.1039/d2cc03752a
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AT muttenthalermarkus expandingtheversatilityandscopeoftheoximeligationrapidbioconjugationtodisulfiderichpeptides

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