Rapid Nucleic Acid Extraction for Aquatic Animal DNA Virus Determination Using Chelex 100 Resin via Conventional PCR and Digital Droplet PCR Detection

SIMPLE SUMMARY: Convenient, fast, and high-quality nucleic acid extraction methods are urgently needed in molecular diagnostic testing for viral pathogens in aquaculture. We developed a viral DNA extraction method from diseased tissues and cells using the Chelex 100 resin solution workflow. The only...

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Detalles Bibliográficos
Autores principales: Hu, Xi, Jiang, Nan, Li, Yiqun, Zhou, Yong, Fan, Yuding, Xue, Mingyang, Zeng, Lingbing, Liu, Wenzhi, Meng, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367309/
https://www.ncbi.nlm.nih.gov/pubmed/35953988
http://dx.doi.org/10.3390/ani12151999

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367309/
https://www.ncbi.nlm.nih.gov/pubmed/35953988
http://dx.doi.org/10.3390/ani12151999

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