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Rapid Nucleic Acid Extraction for Aquatic Animal DNA Virus Determination Using Chelex 100 Resin via Conventional PCR and Digital Droplet PCR Detection
SIMPLE SUMMARY: Convenient, fast, and high-quality nucleic acid extraction methods are urgently needed in molecular diagnostic testing for viral pathogens in aquaculture. We developed a viral DNA extraction method from diseased tissues and cells using the Chelex 100 resin solution workflow. The only...
Autores principales: | Hu, Xi, Jiang, Nan, Li, Yiqun, Zhou, Yong, Fan, Yuding, Xue, Mingyang, Zeng, Lingbing, Liu, Wenzhi, Meng, Yan |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9367309/ https://www.ncbi.nlm.nih.gov/pubmed/35953988 http://dx.doi.org/10.3390/ani12151999 |
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