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Dimethyl Dicarbonate as a Food Additive Effectively Inhibits Geotrichum citri-aurantii of Citrus

Dimethyl dicarbonate (DMDC), a food additive, can be added to a variety of foods as a preservative. This study aimed to evaluate the inhibitory effects of DMDC on Geotrichum citri-aurantii in vitro and in vivo, as well as the potential antifungal mechanism. In vitro experiments showed that 250 mg/L...

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Autores principales: Liu, Shuqi, Zhang, Deyao, Wang, Yuqing, Yang, Fan, Zhao, Juan, Du, Yujie, Tian, Zhonghuan, Long, Chaoan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368502/
https://www.ncbi.nlm.nih.gov/pubmed/35954094
http://dx.doi.org/10.3390/foods11152328
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author Liu, Shuqi
Zhang, Deyao
Wang, Yuqing
Yang, Fan
Zhao, Juan
Du, Yujie
Tian, Zhonghuan
Long, Chaoan
author_facet Liu, Shuqi
Zhang, Deyao
Wang, Yuqing
Yang, Fan
Zhao, Juan
Du, Yujie
Tian, Zhonghuan
Long, Chaoan
author_sort Liu, Shuqi
collection PubMed
description Dimethyl dicarbonate (DMDC), a food additive, can be added to a variety of foods as a preservative. This study aimed to evaluate the inhibitory effects of DMDC on Geotrichum citri-aurantii in vitro and in vivo, as well as the potential antifungal mechanism. In vitro experiments showed that 250 mg/L DMDC completely inhibited the growth of G. citri-aurantii and significantly inhibited spore germination by 96.33%. The relative conductivity and propidium iodide (PI) staining results showed that DMDC at 250 mg/L increased membrane permeability and damaged membrane integrity. Malondialdehyde (MDA) content and 2, 7-Dichlorodihydrofluorescein diacetate (DCHF-DA) staining determination indicated that DMDC resulted in intracellular reactive oxygen species (ROS) accumulation and lipid peroxidation. Scanning electron microscopy (SEM) analysis found that the mycelia were distorted and the surface collapsed after DMDC treatment. Morphological changes in mitochondria and the appearance of cavities were observed by transmission electron microscopy (TEM). In vivo, 500 mg/L DMDC and G. citri-aurantii were inoculated into the wounds of citrus. After 7 days of inoculation, DMDC significantly reduced the disease incidence and disease diameter of sour rot. The storage experiment showed that DMDC treatment did not affect the appearance and quality of fruits. In addition, we found that DMDC at 500 mg/L significantly increased the activity of citrus defense-related enzymes, including peroxidase (POD) and phenylalanine ammonia-lyase (PAL). Therefore, DMDC could be used as an effective method to control citrus sour rot.
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spelling pubmed-93685022022-08-12 Dimethyl Dicarbonate as a Food Additive Effectively Inhibits Geotrichum citri-aurantii of Citrus Liu, Shuqi Zhang, Deyao Wang, Yuqing Yang, Fan Zhao, Juan Du, Yujie Tian, Zhonghuan Long, Chaoan Foods Article Dimethyl dicarbonate (DMDC), a food additive, can be added to a variety of foods as a preservative. This study aimed to evaluate the inhibitory effects of DMDC on Geotrichum citri-aurantii in vitro and in vivo, as well as the potential antifungal mechanism. In vitro experiments showed that 250 mg/L DMDC completely inhibited the growth of G. citri-aurantii and significantly inhibited spore germination by 96.33%. The relative conductivity and propidium iodide (PI) staining results showed that DMDC at 250 mg/L increased membrane permeability and damaged membrane integrity. Malondialdehyde (MDA) content and 2, 7-Dichlorodihydrofluorescein diacetate (DCHF-DA) staining determination indicated that DMDC resulted in intracellular reactive oxygen species (ROS) accumulation and lipid peroxidation. Scanning electron microscopy (SEM) analysis found that the mycelia were distorted and the surface collapsed after DMDC treatment. Morphological changes in mitochondria and the appearance of cavities were observed by transmission electron microscopy (TEM). In vivo, 500 mg/L DMDC and G. citri-aurantii were inoculated into the wounds of citrus. After 7 days of inoculation, DMDC significantly reduced the disease incidence and disease diameter of sour rot. The storage experiment showed that DMDC treatment did not affect the appearance and quality of fruits. In addition, we found that DMDC at 500 mg/L significantly increased the activity of citrus defense-related enzymes, including peroxidase (POD) and phenylalanine ammonia-lyase (PAL). Therefore, DMDC could be used as an effective method to control citrus sour rot. MDPI 2022-08-04 /pmc/articles/PMC9368502/ /pubmed/35954094 http://dx.doi.org/10.3390/foods11152328 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Liu, Shuqi
Zhang, Deyao
Wang, Yuqing
Yang, Fan
Zhao, Juan
Du, Yujie
Tian, Zhonghuan
Long, Chaoan
Dimethyl Dicarbonate as a Food Additive Effectively Inhibits Geotrichum citri-aurantii of Citrus
title Dimethyl Dicarbonate as a Food Additive Effectively Inhibits Geotrichum citri-aurantii of Citrus
title_full Dimethyl Dicarbonate as a Food Additive Effectively Inhibits Geotrichum citri-aurantii of Citrus
title_fullStr Dimethyl Dicarbonate as a Food Additive Effectively Inhibits Geotrichum citri-aurantii of Citrus
title_full_unstemmed Dimethyl Dicarbonate as a Food Additive Effectively Inhibits Geotrichum citri-aurantii of Citrus
title_short Dimethyl Dicarbonate as a Food Additive Effectively Inhibits Geotrichum citri-aurantii of Citrus
title_sort dimethyl dicarbonate as a food additive effectively inhibits geotrichum citri-aurantii of citrus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9368502/
https://www.ncbi.nlm.nih.gov/pubmed/35954094
http://dx.doi.org/10.3390/foods11152328
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