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Preparation of acute midbrain slices containing the superior colliculus and periaqueductal Gray for patch-clamp recordings
This protocol is a practical guide for preparing acute coronal slices from the midbrain of young adult mice for electrophysiology experiments. It describes two different sets of solutions with their respective incubation strategies and two alternative procedures for brain extraction: decapitation un...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371254/ https://www.ncbi.nlm.nih.gov/pubmed/35951507 http://dx.doi.org/10.1371/journal.pone.0271832 |