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Preparation of acute midbrain slices containing the superior colliculus and periaqueductal Gray for patch-clamp recordings

This protocol is a practical guide for preparing acute coronal slices from the midbrain of young adult mice for electrophysiology experiments. It describes two different sets of solutions with their respective incubation strategies and two alternative procedures for brain extraction: decapitation un...

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Detalles Bibliográficos
Autores principales: Pavón Arocas, Oriol, Branco, Tiago
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371254/
https://www.ncbi.nlm.nih.gov/pubmed/35951507
http://dx.doi.org/10.1371/journal.pone.0271832