Enhancement of CRISPR/Cas12a trans-cleavage activity using hairpin DNA reporters

The RNA programmed non-specific (trans) nuclease activity of CRISPR-Cas Type V and VI systems has opened a new era in the field of nucleic acid-based detection. Here, we report on the enhancement of trans-cleavage activity of Cas12a enzymes using hairpin DNA sequences as FRET-based reporters. We dis...

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Detalles Bibliográficos
Autores principales: Rossetti, Marianna, Merlo, Rosa, Bagheri, Neda, Moscone, Danila, Valenti, Anna, Saha, Aakash, Arantes, Pablo R, Ippodrino, Rudy, Ricci, Francesco, Treglia, Ida, Delibato, Elisabetta, van der Oost, John, Palermo, Giulia, Perugino, Giuseppe, Porchetta, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Synthetic Biology and Bioengineering
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371913/
https://www.ncbi.nlm.nih.gov/pubmed/35822842
http://dx.doi.org/10.1093/nar/gkac578

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371913/
https://www.ncbi.nlm.nih.gov/pubmed/35822842
http://dx.doi.org/10.1093/nar/gkac578

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