Cargando…

Enhancement of CRISPR/Cas12a trans-cleavage activity using hairpin DNA reporters

The RNA programmed non-specific (trans) nuclease activity of CRISPR-Cas Type V and VI systems has opened a new era in the field of nucleic acid-based detection. Here, we report on the enhancement of trans-cleavage activity of Cas12a enzymes using hairpin DNA sequences as FRET-based reporters. We dis...

Descripción completa

Detalles Bibliográficos
Autores principales:	Rossetti, Marianna, Merlo, Rosa, Bagheri, Neda, Moscone, Danila, Valenti, Anna, Saha, Aakash, Arantes, Pablo R, Ippodrino, Rudy, Ricci, Francesco, Treglia, Ida, Delibato, Elisabetta, van der Oost, John, Palermo, Giulia, Perugino, Giuseppe, Porchetta, Alessandro
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2022
Materias:	Synthetic Biology and Bioengineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9371913/ https://www.ncbi.nlm.nih.gov/pubmed/35822842 http://dx.doi.org/10.1093/nar/gkac578

Ejemplares similares

A combinatorial approach to the repertoire of RNA kissing motifs; towards multiplex detection by switching hairpin aptamers
por: Durand, Guillaume, et al.
Publicado: (2016)

Genome manipulation by guide-directed Argonaute cleavage
por: Huang, Shan, et al.
Publicado: (2023)

Photomodulating RNA cleavage using photolabile circular antisense oligodeoxynucleotides
por: Tang, XinJing, et al.
Publicado: (2010)

Binary control of enzymatic cleavage of DNA origami by structural antideterminants
por: Stopar, Alex, et al.
Publicado: (2018)

Conditional guide RNA through two intermediate hairpins for programmable CRISPR/Cas9 function: building regulatory connections between endogenous RNA expressions
por: Lin, Jiao, et al.
Publicado: (2020)

TAL nucleases (TALNs): hybrid proteins composed of TAL effectors and FokI DNA-cleavage domain
por: Li, Ting, et al.
Publicado: (2011)

Tandem repeat coupled with endonuclease cleavage (TREC): a seamless modification tool for genome engineering in yeast
por: Noskov, Vladimir N., et al.
Publicado: (2010)

Efficient target cleavage by Type V Cas12a effectors programmed with split CRISPR RNA
por: Shebanova, Regina, et al.
Publicado: (2021)

Pressure modulates the self-cleavage step of the hairpin ribozyme
por: Schuabb, Caroline, et al.
Publicado: (2017)

Engineering naturally occurring trans-acting non-coding RNAs to sense molecular signals
por: Qi, Lei, et al.
Publicado: (2012)

Dystrophin rescue by trans-splicing: a strategy for DMD genotypes not eligible for exon skipping approaches
por: Lorain, Stéphanie, et al.
Publicado: (2013)

Control of ϕC31 integrase-mediated site-specific recombination by protein trans-splicing
por: Olorunniji, Femi J, et al.
Publicado: (2019)

5′-O-Methylphosphonate nucleic acids—new modified DNAs that increase the Escherichia coli RNase H cleavage rate of hybrid duplexes
por: Šípová, Hana, et al.
Publicado: (2014)

FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae
por: Świat, Michal A., et al.
Publicado: (2017)

Double-stranded DNA-templated cleavage of oligonucleotides containing a P3′→N5′ linkage triggered by triplex formation: the effects of chemical modifications and remarkable enhancement in reactivity
por: Ito, Kosuke Ramon, et al.
Publicado: (2010)

Expanding the editable genome and CRISPR–Cas9 versatility using DNA cutting-free gene targeting based on in trans paired nicking
por: Chen, Xiaoyu, et al.
Publicado: (2020)

DNA-Based Nanoswitches: Insights into Electrochemiluminescence Signal Enhancement
por: Zanut, Alessandra, et al.
Publicado: (2021)

Two-step cleavage of hairpin RNA with 5' overhangs by human DICER
por: Ando, Yoshinari, et al.
Publicado: (2011)

Spring loading a pre-cleavage intermediate for hairpin telomere formation
por: Lucyshyn, Danica, et al.
Publicado: (2015)

Streamlined CRISPR genome engineering in wild-type bacteria using SIBR-Cas
por: Patinios, Constantinos, et al.
Publicado: (2021)

Hairpin DNA Sequences Bound Strongly by Bleomycin Exhibit Enhanced Double-Strand Cleavage
por: Roy, Basab, et al.
Publicado: (2014)

Synthetic biology: biology by design
por: Roberts, M. A. J., et al.
Publicado: (2013)

Tuning the dials of Synthetic Biology
por: Arpino, James A. J., et al.
Publicado: (2013)

A novel approach to generate a recombinant toxoid vaccine against Clostridium difficile
por: Donald, Robert G. K., et al.
Publicado: (2013)

Computational protein design with backbone plasticity
por: MacDonald, James T., et al.
Publicado: (2016)

Evolution of DELLA function and signaling in land plants
por: Phokas, Alexandros, et al.
Publicado: (2021)

Recent advances in genetic tools for engineering probiotic lactic acid bacteria
por: Mugwanda, Kanganwiro, et al.
Publicado: (2023)

Fighting COVID-19 Using Molecular Dynamics Simulations
por: Arantes, Pablo R., et al.
Publicado: (2020)

Prolonging genetic circuit stability through adaptive evolution of overlapping genes
por: Chlebek, Jennifer L, et al.
Publicado: (2023)

O(6)-alkylguanine-DNA Alkyltransferases in Microbes Living on the Edge: From Stability to Applicability
por: Mattossovich, Rosanna, et al.
Publicado: (2020)

Allosteric DNA nanoswitches for controlled release of a molecular cargo triggered by biological inputs
por: Rossetti, Marianna, et al.
Publicado: (2017)

Roles of the membrane-reentrant β-hairpin-like loop of RseP protease in selective substrate cleavage
por: Akiyama, Koichiro, et al.
Publicado: (2015)

The telomere resolvase, TelA, utilizes an underwound pre-cleavage intermediate to promote hairpin telomere formation
por: Balouchi, Mahrokh, et al.
Publicado: (2023)

Trans-inhibition of HIV-1 by a long hairpin RNA expressed within the viral genome
por: Konstantinova, Pavlina, et al.
Publicado: (2007)

Production of dumbbell probe through hairpin cleavage-ligation and increasing RCA sensitivity and specificity by circle to circle amplification
por: Wei, Hua, et al.
Publicado: (2016)

Large-Scale Photolithographic Synthesis of Chimeric DNA/RNA Hairpin Microarrays To Explore Sequence Specificity Landscapes of RNase HII Cleavage
por: Lietard, Jory, et al.
Publicado: (2019)

GPU-Enhanced DFTB Metadynamics for Efficiently Predicting Free Energies of Biochemical Systems
por: Kumar, Anshuman, et al.
Publicado: (2023)

Human Sperm Chromosomes: To Form Hairpin-Loops, Or Not to Form Hairpin-Loops, That Is the Question
por: Ioannou, Dimitrios, et al.
Publicado: (2019)

Trinucleotide repeat deletion via a unique hairpin bypass by DNA polymerase β and alternate flap cleavage by flap endonuclease 1
por: Xu, Meng, et al.
Publicado: (2013)

Chiral metallo-supramolecular complexes selectively recognize human telomeric G-quadruplex DNA
por: Yu, Haijia, et al.
Publicado: (2008)

Cannot write session to /tmp/vufind_sessions/sess_479jrm2e322u80eb789hmgikt6