Engineering an adenine base editor in human embryonic stem cells with minimal DNA and RNA off-target activities

Genome editing in pluripotent stem cells (PSCs) using CRISPR technology holds great promise for therapeutic applications. Yet, it has been reported that Cas9-mediated cleavage could cause large deletions or rearrangements of DNA, and the selection of edited PSCs could acquire p53 mutations. Adenine...

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Detalles Bibliográficos
Autores principales: Zhang, Zhenwu, Tao, Wanyu, Huang, Shisheng, Sun, Wenjun, Wang, Yue, Jiang, Wen, Huang, Xingxu, Lin, Chao-Po
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375152/
https://www.ncbi.nlm.nih.gov/pubmed/35991312
http://dx.doi.org/10.1016/j.omtn.2022.07.026

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375152/
https://www.ncbi.nlm.nih.gov/pubmed/35991312
http://dx.doi.org/10.1016/j.omtn.2022.07.026

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