Cargando…
Engineering an adenine base editor in human embryonic stem cells with minimal DNA and RNA off-target activities
Genome editing in pluripotent stem cells (PSCs) using CRISPR technology holds great promise for therapeutic applications. Yet, it has been reported that Cas9-mediated cleavage could cause large deletions or rearrangements of DNA, and the selection of edited PSCs could acquire p53 mutations. Adenine...
Autores principales: | Zhang, Zhenwu, Tao, Wanyu, Huang, Shisheng, Sun, Wenjun, Wang, Yue, Jiang, Wen, Huang, Xingxu, Lin, Chao-Po |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society of Gene & Cell Therapy
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9375152/ https://www.ncbi.nlm.nih.gov/pubmed/35991312 http://dx.doi.org/10.1016/j.omtn.2022.07.026 |
Ejemplares similares
-
Structure-guided engineering of adenine base editor with minimized RNA off-targeting activity
por: Li, Jianan, et al.
Publicado: (2021) -
Engineering of near-PAMless adenine base editor with enhanced editing activity and reduced off-target
por: Cao, Xiaofang, et al.
Publicado: (2022) -
A Cas-embedding strategy for minimizing off-target effects of DNA base editors
por: Liu, Yajing, et al.
Publicado: (2020) -
Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation
por: Wang, Xinjie, et al.
Publicado: (2020) -
Analysis and minimization of cellular RNA editing by DNA adenine base editors
por: Rees, Holly A., et al.
Publicado: (2019)