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Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes

RNA localization and biomolecular condensate formation are key biological strategies for organizing the cytoplasm and generating cellular polarity. In Xenopus oocytes, RNAs required for germ layer patterning localize in biomolecular condensates, termed Localization bodies (L-bodies). Here, we have u...

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Autores principales: Cabral, Sarah E., Otis, Jessica P., Mowry, Kimberly L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9379569/
https://www.ncbi.nlm.nih.gov/pubmed/35982794
http://dx.doi.org/10.1016/j.isci.2022.104811
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author Cabral, Sarah E.
Otis, Jessica P.
Mowry, Kimberly L.
author_facet Cabral, Sarah E.
Otis, Jessica P.
Mowry, Kimberly L.
author_sort Cabral, Sarah E.
collection PubMed
description RNA localization and biomolecular condensate formation are key biological strategies for organizing the cytoplasm and generating cellular polarity. In Xenopus oocytes, RNAs required for germ layer patterning localize in biomolecular condensates, termed Localization bodies (L-bodies). Here, we have used an L-body RNA-binding protein, PTBP3, to test the role of RNA–protein interactions in regulating the biophysical characteristics of L-bodies in vivo and PTBP3–RNA condensates in vitro. Our results reveal that RNA–protein interactions drive recruitment of PTBP3 and localized RNA to L-bodies and that multivalent interactions tune the dynamics of the PTBP3 after localization. In a concentration-dependent manner, RNA becomes non-dynamic and interactions with the RNA determine PTBP3 dynamics within these biomolecular condensates in vivo and in vitro. Importantly, RNA, and not protein, is required for maintenance of the PTBP3–RNA condensates in vitro, pointing to a model where RNA serves as a non-dynamic substructure in these condensates.
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spelling pubmed-93795692022-08-17 Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes Cabral, Sarah E. Otis, Jessica P. Mowry, Kimberly L. iScience Article RNA localization and biomolecular condensate formation are key biological strategies for organizing the cytoplasm and generating cellular polarity. In Xenopus oocytes, RNAs required for germ layer patterning localize in biomolecular condensates, termed Localization bodies (L-bodies). Here, we have used an L-body RNA-binding protein, PTBP3, to test the role of RNA–protein interactions in regulating the biophysical characteristics of L-bodies in vivo and PTBP3–RNA condensates in vitro. Our results reveal that RNA–protein interactions drive recruitment of PTBP3 and localized RNA to L-bodies and that multivalent interactions tune the dynamics of the PTBP3 after localization. In a concentration-dependent manner, RNA becomes non-dynamic and interactions with the RNA determine PTBP3 dynamics within these biomolecular condensates in vivo and in vitro. Importantly, RNA, and not protein, is required for maintenance of the PTBP3–RNA condensates in vitro, pointing to a model where RNA serves as a non-dynamic substructure in these condensates. Elsevier 2022-07-21 /pmc/articles/PMC9379569/ /pubmed/35982794 http://dx.doi.org/10.1016/j.isci.2022.104811 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Cabral, Sarah E.
Otis, Jessica P.
Mowry, Kimberly L.
Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes
title Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes
title_full Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes
title_fullStr Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes
title_full_unstemmed Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes
title_short Multivalent interactions with RNA drive recruitment and dynamics in biomolecular condensates in Xenopus oocytes
title_sort multivalent interactions with rna drive recruitment and dynamics in biomolecular condensates in xenopus oocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9379569/
https://www.ncbi.nlm.nih.gov/pubmed/35982794
http://dx.doi.org/10.1016/j.isci.2022.104811
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