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Molecular mapping of QTLs for grain dimension traits in Basmati rice

Basmati rice is known for its extra-long slender grains, exceptional kernel dimensions after cooking, high volume expansion, and strong aroma. Developing high yielding Basmati rice varieties with good cooking quality is a gigantic task. Therefore, identifying the genomic regions governing the grain...

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Autores principales: Malik, Ankit, Kumar, Aruna, Ellur, Ranjith Kumar, Krishnan S, Gopala, Dixit, Deepshikha, Bollinedi, Haritha, Vinod, KK, Nagarajan, M, Bhowmick, PK, Singh, NK, Singh, AK
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9379801/
https://www.ncbi.nlm.nih.gov/pubmed/35983411
http://dx.doi.org/10.3389/fgene.2022.932166
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author Malik, Ankit
Kumar, Aruna
Ellur, Ranjith Kumar
Krishnan S, Gopala
Dixit, Deepshikha
Bollinedi, Haritha
Vinod, KK
Nagarajan, M
Bhowmick, PK
Singh, NK
Singh, AK
author_facet Malik, Ankit
Kumar, Aruna
Ellur, Ranjith Kumar
Krishnan S, Gopala
Dixit, Deepshikha
Bollinedi, Haritha
Vinod, KK
Nagarajan, M
Bhowmick, PK
Singh, NK
Singh, AK
author_sort Malik, Ankit
collection PubMed
description Basmati rice is known for its extra-long slender grains, exceptional kernel dimensions after cooking, high volume expansion, and strong aroma. Developing high yielding Basmati rice varieties with good cooking quality is a gigantic task. Therefore, identifying the genomic regions governing the grain and cooked kernel dimension traits is of utmost importance for its use in marker-assisted breeding. Although several QTLs governing grain dimension traits have been reported, limited attempts have been made to map QTLs for grain and cooked kernel dimension traits of Basmati rice. In the current study, a population of recombinant inbred lines (RIL) was generated from a cross of Sonasal and Pusa Basmati 1121 (PB1121). In the RIL population, there was a significant positive correlation among the length (RRL: rough rice length, MRL: milled rice length, CKL: cooked kernel length) and breadth (RRB: rough rice breadth, MRB: milled rice breadth and CKB: cooked kernel breadth) of the related traits, while there was significant negative correlation between them. QTL mapping has led to the identification of four major genomic regions governing MRL and CKL. Two QTLs co-localize with the earlier reported major gene GS3 and a QTL qGRL7.1, while the remaining two QTLs viz., qCKL3.2 (qMRL3.2) and qCKL4.1 (qMRL4.1) were novel. The QTL qCKL3.2 has been bracketed to a genomic region of 0.78 Mb between the markers RM15247 and RM15281. Annotation of this region identified 18 gene models, of which the genes predicted to encode pentatricopeptides and brassinosteroid insensitive 1-associated receptor kinase 1 precursor may be the putative candidate genes. Furthermore, we identified a novel QTL qKER2.1 governing kernel elongation ratio (KER) in Basmati rice.
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spelling pubmed-93798012022-08-17 Molecular mapping of QTLs for grain dimension traits in Basmati rice Malik, Ankit Kumar, Aruna Ellur, Ranjith Kumar Krishnan S, Gopala Dixit, Deepshikha Bollinedi, Haritha Vinod, KK Nagarajan, M Bhowmick, PK Singh, NK Singh, AK Front Genet Genetics Basmati rice is known for its extra-long slender grains, exceptional kernel dimensions after cooking, high volume expansion, and strong aroma. Developing high yielding Basmati rice varieties with good cooking quality is a gigantic task. Therefore, identifying the genomic regions governing the grain and cooked kernel dimension traits is of utmost importance for its use in marker-assisted breeding. Although several QTLs governing grain dimension traits have been reported, limited attempts have been made to map QTLs for grain and cooked kernel dimension traits of Basmati rice. In the current study, a population of recombinant inbred lines (RIL) was generated from a cross of Sonasal and Pusa Basmati 1121 (PB1121). In the RIL population, there was a significant positive correlation among the length (RRL: rough rice length, MRL: milled rice length, CKL: cooked kernel length) and breadth (RRB: rough rice breadth, MRB: milled rice breadth and CKB: cooked kernel breadth) of the related traits, while there was significant negative correlation between them. QTL mapping has led to the identification of four major genomic regions governing MRL and CKL. Two QTLs co-localize with the earlier reported major gene GS3 and a QTL qGRL7.1, while the remaining two QTLs viz., qCKL3.2 (qMRL3.2) and qCKL4.1 (qMRL4.1) were novel. The QTL qCKL3.2 has been bracketed to a genomic region of 0.78 Mb between the markers RM15247 and RM15281. Annotation of this region identified 18 gene models, of which the genes predicted to encode pentatricopeptides and brassinosteroid insensitive 1-associated receptor kinase 1 precursor may be the putative candidate genes. Furthermore, we identified a novel QTL qKER2.1 governing kernel elongation ratio (KER) in Basmati rice. Frontiers Media S.A. 2022-08-02 /pmc/articles/PMC9379801/ /pubmed/35983411 http://dx.doi.org/10.3389/fgene.2022.932166 Text en Copyright © 2022 Malik, Kumar, Ellur, Krishnan S, Dixit, Bollinedi, Vinod, Nagarajan, Bhowmick, Singh and Singh. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Malik, Ankit
Kumar, Aruna
Ellur, Ranjith Kumar
Krishnan S, Gopala
Dixit, Deepshikha
Bollinedi, Haritha
Vinod, KK
Nagarajan, M
Bhowmick, PK
Singh, NK
Singh, AK
Molecular mapping of QTLs for grain dimension traits in Basmati rice
title Molecular mapping of QTLs for grain dimension traits in Basmati rice
title_full Molecular mapping of QTLs for grain dimension traits in Basmati rice
title_fullStr Molecular mapping of QTLs for grain dimension traits in Basmati rice
title_full_unstemmed Molecular mapping of QTLs for grain dimension traits in Basmati rice
title_short Molecular mapping of QTLs for grain dimension traits in Basmati rice
title_sort molecular mapping of qtls for grain dimension traits in basmati rice
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9379801/
https://www.ncbi.nlm.nih.gov/pubmed/35983411
http://dx.doi.org/10.3389/fgene.2022.932166
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