Development of in vivo HDX‐MS with applications to a TonB‐dependent transporter and other proteins

Hydrogen‐deuterium exchange mass spectrometry (HDX‐MS) is a powerful tool that monitors protein dynamics in solution. However, the reversible nature of HDX labels has largely limited the application to in vitro systems. Here, we describe a protocol for measuring HDX‐MS in living Escherichia coli cel...

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Detalles Bibliográficos
Autores principales: Lin, Xiaoxuan, Zmyslowski, Adam M., Gagnon, Isabelle A., Nakamoto, Robert K., Sosnick, Tobin R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley & Sons, Inc. 2022
Materias:
Methods and Applications
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382693/
https://www.ncbi.nlm.nih.gov/pubmed/36040258
http://dx.doi.org/10.1002/pro.4402

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382693/
https://www.ncbi.nlm.nih.gov/pubmed/36040258
http://dx.doi.org/10.1002/pro.4402

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