Human growth hormone inclusion bodies present native-like secondary and tertiary structures which can be preserved by mild solubilization for refolding

BACKGROUND: Native-like secondary structures and biological activity have been described for proteins in inclusion bodies (IBs). Tertiary structure analysis, however, is hampered due to the necessity of mild solubilization conditions. Denaturing reagents used for IBs solubilization generally lead to...

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Detalles Bibliográficos
Autores principales: Chura-Chambi, Rosa Maria, Farah, Chuck Shaker, Morganti, Ligia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382763/
https://www.ncbi.nlm.nih.gov/pubmed/35978337
http://dx.doi.org/10.1186/s12934-022-01887-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9382763/
https://www.ncbi.nlm.nih.gov/pubmed/35978337
http://dx.doi.org/10.1186/s12934-022-01887-1

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