Large scale screening of CRISPR guide RNAs using an optimized high throughput robotics system

All CRISPR/CAS systems utilize CRISPR guide RNAs (crRNAs), the design of which depend on the type of CAS protein, genetic target and the environment/matrix. While machine learning approaches have recently been developed to optimize some crRNA designs, candidate crRNAs must still be screened for effi...

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Detalles Bibliográficos
Autores principales: Spangler, J. R., Leski, T. A., Schultzhaus, Z., Wang, Z., Stenger, D. A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385653/
https://www.ncbi.nlm.nih.gov/pubmed/35977955
http://dx.doi.org/10.1038/s41598-022-17474-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9385653/
https://www.ncbi.nlm.nih.gov/pubmed/35977955
http://dx.doi.org/10.1038/s41598-022-17474-8

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