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High-content analysis of neuronal morphology in human iPSC-derived neurons

We present a high-content analysis (HCA) protocol for monitoring the outgrowth capacity of human neurons derived from induced pluripotent stem cells (iPSCs). We describe steps to perform HCA imaging, followed by quantifying the morphology of dendrites and axons within a high-throughput system to eva...

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Detalles Bibliográficos
Autores principales: Lickfett, Selene, Menacho, Carmen, Zink, Annika, Telugu, Narasimha Swamy, Beller, Mathias, Diecke, Sebastian, Cambridge, Sidney, Prigione, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9386098/
https://www.ncbi.nlm.nih.gov/pubmed/35990743
http://dx.doi.org/10.1016/j.xpro.2022.101567