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Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)

BACKGROUND: High cost of commercial RNA extraction kits limits the testing efficiency of SARS-CoV-2. Here, we developed a simple nucleic acid extraction method for the detection of SARS-CoV-2 directly from nasopharyngeal swab samples. METHODS: A pH sensitive dye was used as the end point detection m...

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Detalles Bibliográficos
Autores principales: Lai, Meng Yee, Bukhari, Fatma Diyana Mohd, Zulkefli, Nur Zulaikha, Ismail, Ilyiana, Mustapa, Nur Izati, Soh, Tuan Suhaila Tuan, Hassan, Afifah Haji, Peariasamy, Kalaiarasu M., Lee, Yee Leng, Suppiah, Jeyanthi, Thayan, Ravindran, Isa, Mohd Khairi Mat, Wahid, Nur Zafirah Abdul, Lau, Yee Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9387413/
https://www.ncbi.nlm.nih.gov/pubmed/35982419
http://dx.doi.org/10.1186/s12879-022-07684-w