Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)

BACKGROUND: High cost of commercial RNA extraction kits limits the testing efficiency of SARS-CoV-2. Here, we developed a simple nucleic acid extraction method for the detection of SARS-CoV-2 directly from nasopharyngeal swab samples. METHODS: A pH sensitive dye was used as the end point detection m...

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Autores principales: Lai, Meng Yee, Bukhari, Fatma Diyana Mohd, Zulkefli, Nur Zulaikha, Ismail, Ilyiana, Mustapa, Nur Izati, Soh, Tuan Suhaila Tuan, Hassan, Afifah Haji, Peariasamy, Kalaiarasu M., Lee, Yee Leng, Suppiah, Jeyanthi, Thayan, Ravindran, Isa, Mohd Khairi Mat, Wahid, Nur Zafirah Abdul, Lau, Yee Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9387413/
https://www.ncbi.nlm.nih.gov/pubmed/35982419
http://dx.doi.org/10.1186/s12879-022-07684-w
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author Lai, Meng Yee
Bukhari, Fatma Diyana Mohd
Zulkefli, Nur Zulaikha
Ismail, Ilyiana
Mustapa, Nur Izati
Soh, Tuan Suhaila Tuan
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M.
Lee, Yee Leng
Suppiah, Jeyanthi
Thayan, Ravindran
Isa, Mohd Khairi Mat
Wahid, Nur Zafirah Abdul
Lau, Yee Ling
author_facet Lai, Meng Yee
Bukhari, Fatma Diyana Mohd
Zulkefli, Nur Zulaikha
Ismail, Ilyiana
Mustapa, Nur Izati
Soh, Tuan Suhaila Tuan
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M.
Lee, Yee Leng
Suppiah, Jeyanthi
Thayan, Ravindran
Isa, Mohd Khairi Mat
Wahid, Nur Zafirah Abdul
Lau, Yee Ling
author_sort Lai, Meng Yee
collection PubMed
description BACKGROUND: High cost of commercial RNA extraction kits limits the testing efficiency of SARS-CoV-2. Here, we developed a simple nucleic acid extraction method for the detection of SARS-CoV-2 directly from nasopharyngeal swab samples. METHODS: A pH sensitive dye was used as the end point detection method. The obvious colour changes between positive and negative reactions eliminates the need of other equipment. RESULTS: Clinical testing using 260 samples showed 92.7% sensitivity (95% CI 87.3–96.3%) and 93.6% specificity (95% CI 87.3–97.4%) of RT-LAMP. CONCLUSIONS: The simple RNA extraction method minimizes the need for any extensive laboratory set-up. We suggest combining this simple nucleic acid extraction method and RT-LAMP technology as the point-of care diagnostic tool. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-022-07684-w.
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spelling pubmed-93874132022-08-18 Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP) Lai, Meng Yee Bukhari, Fatma Diyana Mohd Zulkefli, Nur Zulaikha Ismail, Ilyiana Mustapa, Nur Izati Soh, Tuan Suhaila Tuan Hassan, Afifah Haji Peariasamy, Kalaiarasu M. Lee, Yee Leng Suppiah, Jeyanthi Thayan, Ravindran Isa, Mohd Khairi Mat Wahid, Nur Zafirah Abdul Lau, Yee Ling BMC Infect Dis Research BACKGROUND: High cost of commercial RNA extraction kits limits the testing efficiency of SARS-CoV-2. Here, we developed a simple nucleic acid extraction method for the detection of SARS-CoV-2 directly from nasopharyngeal swab samples. METHODS: A pH sensitive dye was used as the end point detection method. The obvious colour changes between positive and negative reactions eliminates the need of other equipment. RESULTS: Clinical testing using 260 samples showed 92.7% sensitivity (95% CI 87.3–96.3%) and 93.6% specificity (95% CI 87.3–97.4%) of RT-LAMP. CONCLUSIONS: The simple RNA extraction method minimizes the need for any extensive laboratory set-up. We suggest combining this simple nucleic acid extraction method and RT-LAMP technology as the point-of care diagnostic tool. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12879-022-07684-w. BioMed Central 2022-08-18 /pmc/articles/PMC9387413/ /pubmed/35982419 http://dx.doi.org/10.1186/s12879-022-07684-w Text en © The Author(s) 2022, corrected publication 2023 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Lai, Meng Yee
Bukhari, Fatma Diyana Mohd
Zulkefli, Nur Zulaikha
Ismail, Ilyiana
Mustapa, Nur Izati
Soh, Tuan Suhaila Tuan
Hassan, Afifah Haji
Peariasamy, Kalaiarasu M.
Lee, Yee Leng
Suppiah, Jeyanthi
Thayan, Ravindran
Isa, Mohd Khairi Mat
Wahid, Nur Zafirah Abdul
Lau, Yee Ling
Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)
title Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)
title_full Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)
title_fullStr Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)
title_full_unstemmed Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)
title_short Clinical testing on SARS-CoV-2 swab samples using reverse-transcription loop-mediated isothermal amplification (RT-LAMP)
title_sort clinical testing on sars-cov-2 swab samples using reverse-transcription loop-mediated isothermal amplification (rt-lamp)
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9387413/
https://www.ncbi.nlm.nih.gov/pubmed/35982419
http://dx.doi.org/10.1186/s12879-022-07684-w
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