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Protocol for the generation of HIV-1 genomic RNA with altered levels of N(6)-methyladenosine

N(6)-methyladenosine (m(6)A) modification of human immunodeficiency virus type 1 (HIV-1) RNA plays a critical role in regulating viral replication and evasion of innate immunity. Here, we describe a protocol for the production of HIV-1 with altered m(6)A levels by manipulating the expression of m(6)...

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Detalles Bibliográficos
Autores principales: Phillips, Stacia, Baek, Alice, Kim, Sanggu, Chen, Shuliang, Wu, Li
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9389413/
https://www.ncbi.nlm.nih.gov/pubmed/35990737
http://dx.doi.org/10.1016/j.xpro.2022.101616