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Rapid detection of SARS-CoV-2 variants of concern by single nucleotide polymorphism genotyping using TaqMan assays

We evaluated the performance of SARS-CoV-2 TaqMan real-time reverse-transcription PCR (RT-qPCR) assays (ThermoFisher) for detecting 2 nonsynonymous spike protein mutations, E484K and N501Y. Assay accuracy was evaluated by whole genome sequencing (WGS). Residual nasopharyngeal SARS-CoV-2 positive sam...

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Detalles Bibliográficos
Autores principales: Velu, Priya, Cong, Lin, Rand, Sophie, Qiu, Yuqing, Zhang, Zhengmao, Zhang, Jianxuan, Guo, Jianfen, Ruggiero, Phyllis, Sukhu, Ashley, Fauntleroy, Kathy, Imada, Eddie, Zanettini, Claudio, Brundage, David, Westblade, Lars, Marchionni, Luigi, Cushing, Melissa M., Rennert, Hanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9392658/
https://www.ncbi.nlm.nih.gov/pubmed/36122486
http://dx.doi.org/10.1016/j.diagmicrobio.2022.115789