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A versatile enhanced freeze-substitution protocol for volume electron microscopy

Volume electron microscopy, a powerful approach to generate large three-dimensional cell and tissue volumes at electron microscopy resolutions, is rapidly becoming a routine tool for understanding fundamental and applied biological questions. One of the enabling factors for its adoption has been the...

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Detalles Bibliográficos
Autores principales: Bélanger, Sébastien, Berensmann, Heather, Baena, Valentina, Duncan, Keith, Meyers, Blake C., Narayan, Kedar, Czymmek, Kirk J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9393620/
https://www.ncbi.nlm.nih.gov/pubmed/36003147
http://dx.doi.org/10.3389/fcell.2022.933376