Establishment of a DNA-free genome editing and protoplast regeneration method in cultivated tomato (Solanum lycopersicum)

KEY MESSAGE: We have established a DNA-free genome editing method via ribonucleoprotein-based CRISPR/Cas9 in cultivated tomato and obtained mutant plants regenerated from transfected protoplasts with a high mutation rate. ABSTRACT: The application of genome editing as a research and breeding method...

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Detalles Bibliográficos
Autores principales: Liu, Ying, Andersson, Mariette, Granell, Antonio, Cardi, Teodoro, Hofvander, Per, Nicolia, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395478/
https://www.ncbi.nlm.nih.gov/pubmed/35773498
http://dx.doi.org/10.1007/s00299-022-02893-8

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9395478/
https://www.ncbi.nlm.nih.gov/pubmed/35773498
http://dx.doi.org/10.1007/s00299-022-02893-8

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