The Golgi complex: An organelle that determines urothelial cell biology in health and disease

The Golgi complex undergoes considerable structural remodeling during differentiation of urothelial cells in vivo and in vitro. It is known that in a healthy bladder the differentiation from the basal to the superficial cell layer leads to the formation of the tightest barrier in our body, i.e., the...

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Autores principales: Kreft, Mateja Erdani, Mironov, Alexander A., Hudoklin, Samo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399047/
https://www.ncbi.nlm.nih.gov/pubmed/35773494
http://dx.doi.org/10.1007/s00418-022-02121-0
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author Kreft, Mateja Erdani
Mironov, Alexander A.
Hudoklin, Samo
author_facet Kreft, Mateja Erdani
Mironov, Alexander A.
Hudoklin, Samo
author_sort Kreft, Mateja Erdani
collection PubMed
description The Golgi complex undergoes considerable structural remodeling during differentiation of urothelial cells in vivo and in vitro. It is known that in a healthy bladder the differentiation from the basal to the superficial cell layer leads to the formation of the tightest barrier in our body, i.e., the blood–urine barrier. In this process, urothelial cells start expressing tight junctional proteins, apical membrane lipids, surface glycans, and integral membrane proteins, the uroplakins (UPs). The latter are the most abundant membrane proteins in the apical plasma membrane of differentiated superficial urothelial cells (UCs) and, in addition to well-developed tight junctions, contribute to the permeability barrier by their structural organization and by hindering endocytosis from the apical plasma membrane. By studying the transport of UPs, we were able to demonstrate their differentiation-dependent effect on the Golgi architecture. Although fragmentation of the Golgi complex is known to be associated with mitosis and apoptosis, we found that the process of Golgi fragmentation is required for delivery of certain specific urothelial differentiation cargoes to the plasma membrane as well as for cell–cell communication. In this review, we will discuss the currently known contribution of the Golgi complex to the formation of the blood–urine barrier in normal UCs and how it may be involved in the loss of the blood–urine barrier in cancer. Some open questions related to the Golgi complex in the urothelium will be highlighted.
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spelling pubmed-93990472022-08-25 The Golgi complex: An organelle that determines urothelial cell biology in health and disease Kreft, Mateja Erdani Mironov, Alexander A. Hudoklin, Samo Histochem Cell Biol Original Paper The Golgi complex undergoes considerable structural remodeling during differentiation of urothelial cells in vivo and in vitro. It is known that in a healthy bladder the differentiation from the basal to the superficial cell layer leads to the formation of the tightest barrier in our body, i.e., the blood–urine barrier. In this process, urothelial cells start expressing tight junctional proteins, apical membrane lipids, surface glycans, and integral membrane proteins, the uroplakins (UPs). The latter are the most abundant membrane proteins in the apical plasma membrane of differentiated superficial urothelial cells (UCs) and, in addition to well-developed tight junctions, contribute to the permeability barrier by their structural organization and by hindering endocytosis from the apical plasma membrane. By studying the transport of UPs, we were able to demonstrate their differentiation-dependent effect on the Golgi architecture. Although fragmentation of the Golgi complex is known to be associated with mitosis and apoptosis, we found that the process of Golgi fragmentation is required for delivery of certain specific urothelial differentiation cargoes to the plasma membrane as well as for cell–cell communication. In this review, we will discuss the currently known contribution of the Golgi complex to the formation of the blood–urine barrier in normal UCs and how it may be involved in the loss of the blood–urine barrier in cancer. Some open questions related to the Golgi complex in the urothelium will be highlighted. Springer Berlin Heidelberg 2022-06-30 2022 /pmc/articles/PMC9399047/ /pubmed/35773494 http://dx.doi.org/10.1007/s00418-022-02121-0 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Paper
Kreft, Mateja Erdani
Mironov, Alexander A.
Hudoklin, Samo
The Golgi complex: An organelle that determines urothelial cell biology in health and disease
title The Golgi complex: An organelle that determines urothelial cell biology in health and disease
title_full The Golgi complex: An organelle that determines urothelial cell biology in health and disease
title_fullStr The Golgi complex: An organelle that determines urothelial cell biology in health and disease
title_full_unstemmed The Golgi complex: An organelle that determines urothelial cell biology in health and disease
title_short The Golgi complex: An organelle that determines urothelial cell biology in health and disease
title_sort golgi complex: an organelle that determines urothelial cell biology in health and disease
topic Original Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399047/
https://www.ncbi.nlm.nih.gov/pubmed/35773494
http://dx.doi.org/10.1007/s00418-022-02121-0
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