An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration
Stem cell-based transplantation is a promising therapeutic approach for intervertebral disc degeneration (IDD). Current limitations of stem cells include with their insufficient cell source, poor proliferation capacity, low nucleus pulposus (NP)-specific differentiation potential, and inability to a...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399388/ https://www.ncbi.nlm.nih.gov/pubmed/36017070 http://dx.doi.org/10.1016/j.bioactmat.2022.07.024 |
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author | Xia, Kai-shun Li, Dong-dong Wang, Cheng-gui Ying, Li-wei Wang, Jing-kai Yang, Biao Shu, Jia-wei Huang, Xian-peng Zhang, Yu-ang Yu, Chao Zhou, Xiao-peng Li, Fang-cai Slater, Nigel K.H. Tang, Jian-bin Chen, Qi-xin Liang, Cheng-zhen |
author_facet | Xia, Kai-shun Li, Dong-dong Wang, Cheng-gui Ying, Li-wei Wang, Jing-kai Yang, Biao Shu, Jia-wei Huang, Xian-peng Zhang, Yu-ang Yu, Chao Zhou, Xiao-peng Li, Fang-cai Slater, Nigel K.H. Tang, Jian-bin Chen, Qi-xin Liang, Cheng-zhen |
author_sort | Xia, Kai-shun |
collection | PubMed |
description | Stem cell-based transplantation is a promising therapeutic approach for intervertebral disc degeneration (IDD). Current limitations of stem cells include with their insufficient cell source, poor proliferation capacity, low nucleus pulposus (NP)-specific differentiation potential, and inability to avoid pyroptosis caused by the acidic IDD microenvironment after transplantation. To address these challenges, embryo-derived long-term expandable nucleus pulposus progenitor cells (NPPCs) and esterase-responsive ibuprofen nano-micelles (PEG-PIB) were prepared for synergistic transplantation. In this study, we propose a biomaterial pre-modification cell strategy; the PEG-PIB were endocytosed to pre-modify the NPPCs with adaptability in harsh IDD microenvironment through inhibiting pyroptosis. The results indicated that the PEG-PIB pre-modified NPPCs exhibited inhibition of pyroptosis in vitro; their further synergistic transplantation yielded effective functional recovery, histological regeneration, and inhibition of pyroptosis during IDD regeneration. Herein, we offer a novel biomaterial pre-modification cell strategy for synergistic transplantation with promising therapeutic effects in IDD regeneration. |
format | Online Article Text |
id | pubmed-9399388 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | KeAi Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-93993882022-08-24 An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration Xia, Kai-shun Li, Dong-dong Wang, Cheng-gui Ying, Li-wei Wang, Jing-kai Yang, Biao Shu, Jia-wei Huang, Xian-peng Zhang, Yu-ang Yu, Chao Zhou, Xiao-peng Li, Fang-cai Slater, Nigel K.H. Tang, Jian-bin Chen, Qi-xin Liang, Cheng-zhen Bioact Mater Article Stem cell-based transplantation is a promising therapeutic approach for intervertebral disc degeneration (IDD). Current limitations of stem cells include with their insufficient cell source, poor proliferation capacity, low nucleus pulposus (NP)-specific differentiation potential, and inability to avoid pyroptosis caused by the acidic IDD microenvironment after transplantation. To address these challenges, embryo-derived long-term expandable nucleus pulposus progenitor cells (NPPCs) and esterase-responsive ibuprofen nano-micelles (PEG-PIB) were prepared for synergistic transplantation. In this study, we propose a biomaterial pre-modification cell strategy; the PEG-PIB were endocytosed to pre-modify the NPPCs with adaptability in harsh IDD microenvironment through inhibiting pyroptosis. The results indicated that the PEG-PIB pre-modified NPPCs exhibited inhibition of pyroptosis in vitro; their further synergistic transplantation yielded effective functional recovery, histological regeneration, and inhibition of pyroptosis during IDD regeneration. Herein, we offer a novel biomaterial pre-modification cell strategy for synergistic transplantation with promising therapeutic effects in IDD regeneration. KeAi Publishing 2022-08-14 /pmc/articles/PMC9399388/ /pubmed/36017070 http://dx.doi.org/10.1016/j.bioactmat.2022.07.024 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Article Xia, Kai-shun Li, Dong-dong Wang, Cheng-gui Ying, Li-wei Wang, Jing-kai Yang, Biao Shu, Jia-wei Huang, Xian-peng Zhang, Yu-ang Yu, Chao Zhou, Xiao-peng Li, Fang-cai Slater, Nigel K.H. Tang, Jian-bin Chen, Qi-xin Liang, Cheng-zhen An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration |
title | An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration |
title_full | An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration |
title_fullStr | An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration |
title_full_unstemmed | An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration |
title_short | An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration |
title_sort | esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399388/ https://www.ncbi.nlm.nih.gov/pubmed/36017070 http://dx.doi.org/10.1016/j.bioactmat.2022.07.024 |
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