An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration

Stem cell-based transplantation is a promising therapeutic approach for intervertebral disc degeneration (IDD). Current limitations of stem cells include with their insufficient cell source, poor proliferation capacity, low nucleus pulposus (NP)-specific differentiation potential, and inability to a...

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Autores principales: Xia, Kai-shun, Li, Dong-dong, Wang, Cheng-gui, Ying, Li-wei, Wang, Jing-kai, Yang, Biao, Shu, Jia-wei, Huang, Xian-peng, Zhang, Yu-ang, Yu, Chao, Zhou, Xiao-peng, Li, Fang-cai, Slater, Nigel K.H., Tang, Jian-bin, Chen, Qi-xin, Liang, Cheng-zhen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: KeAi Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399388/
https://www.ncbi.nlm.nih.gov/pubmed/36017070
http://dx.doi.org/10.1016/j.bioactmat.2022.07.024
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author Xia, Kai-shun
Li, Dong-dong
Wang, Cheng-gui
Ying, Li-wei
Wang, Jing-kai
Yang, Biao
Shu, Jia-wei
Huang, Xian-peng
Zhang, Yu-ang
Yu, Chao
Zhou, Xiao-peng
Li, Fang-cai
Slater, Nigel K.H.
Tang, Jian-bin
Chen, Qi-xin
Liang, Cheng-zhen
author_facet Xia, Kai-shun
Li, Dong-dong
Wang, Cheng-gui
Ying, Li-wei
Wang, Jing-kai
Yang, Biao
Shu, Jia-wei
Huang, Xian-peng
Zhang, Yu-ang
Yu, Chao
Zhou, Xiao-peng
Li, Fang-cai
Slater, Nigel K.H.
Tang, Jian-bin
Chen, Qi-xin
Liang, Cheng-zhen
author_sort Xia, Kai-shun
collection PubMed
description Stem cell-based transplantation is a promising therapeutic approach for intervertebral disc degeneration (IDD). Current limitations of stem cells include with their insufficient cell source, poor proliferation capacity, low nucleus pulposus (NP)-specific differentiation potential, and inability to avoid pyroptosis caused by the acidic IDD microenvironment after transplantation. To address these challenges, embryo-derived long-term expandable nucleus pulposus progenitor cells (NPPCs) and esterase-responsive ibuprofen nano-micelles (PEG-PIB) were prepared for synergistic transplantation. In this study, we propose a biomaterial pre-modification cell strategy; the PEG-PIB were endocytosed to pre-modify the NPPCs with adaptability in harsh IDD microenvironment through inhibiting pyroptosis. The results indicated that the PEG-PIB pre-modified NPPCs exhibited inhibition of pyroptosis in vitro; their further synergistic transplantation yielded effective functional recovery, histological regeneration, and inhibition of pyroptosis during IDD regeneration. Herein, we offer a novel biomaterial pre-modification cell strategy for synergistic transplantation with promising therapeutic effects in IDD regeneration.
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spelling pubmed-93993882022-08-24 An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration Xia, Kai-shun Li, Dong-dong Wang, Cheng-gui Ying, Li-wei Wang, Jing-kai Yang, Biao Shu, Jia-wei Huang, Xian-peng Zhang, Yu-ang Yu, Chao Zhou, Xiao-peng Li, Fang-cai Slater, Nigel K.H. Tang, Jian-bin Chen, Qi-xin Liang, Cheng-zhen Bioact Mater Article Stem cell-based transplantation is a promising therapeutic approach for intervertebral disc degeneration (IDD). Current limitations of stem cells include with their insufficient cell source, poor proliferation capacity, low nucleus pulposus (NP)-specific differentiation potential, and inability to avoid pyroptosis caused by the acidic IDD microenvironment after transplantation. To address these challenges, embryo-derived long-term expandable nucleus pulposus progenitor cells (NPPCs) and esterase-responsive ibuprofen nano-micelles (PEG-PIB) were prepared for synergistic transplantation. In this study, we propose a biomaterial pre-modification cell strategy; the PEG-PIB were endocytosed to pre-modify the NPPCs with adaptability in harsh IDD microenvironment through inhibiting pyroptosis. The results indicated that the PEG-PIB pre-modified NPPCs exhibited inhibition of pyroptosis in vitro; their further synergistic transplantation yielded effective functional recovery, histological regeneration, and inhibition of pyroptosis during IDD regeneration. Herein, we offer a novel biomaterial pre-modification cell strategy for synergistic transplantation with promising therapeutic effects in IDD regeneration. KeAi Publishing 2022-08-14 /pmc/articles/PMC9399388/ /pubmed/36017070 http://dx.doi.org/10.1016/j.bioactmat.2022.07.024 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Xia, Kai-shun
Li, Dong-dong
Wang, Cheng-gui
Ying, Li-wei
Wang, Jing-kai
Yang, Biao
Shu, Jia-wei
Huang, Xian-peng
Zhang, Yu-ang
Yu, Chao
Zhou, Xiao-peng
Li, Fang-cai
Slater, Nigel K.H.
Tang, Jian-bin
Chen, Qi-xin
Liang, Cheng-zhen
An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration
title An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration
title_full An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration
title_fullStr An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration
title_full_unstemmed An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration
title_short An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration
title_sort esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9399388/
https://www.ncbi.nlm.nih.gov/pubmed/36017070
http://dx.doi.org/10.1016/j.bioactmat.2022.07.024
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