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An optimized proximity ligation assay to detect telomere dysfunction induced foci in human and mouse cells

Telomere dysfunction-induced foci (TIF) can be measured by immunofluorescence, combined with telomere-fluorescent in situ hybridization. We modified this approach by combining the proximity ligation assay (PLA), which detects colocalization of two molecules in proximity through a signal amplificatio...

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Detalles Bibliográficos
Autores principales:	Wang, Yajun, Ferrucci, Luigi, Seidman, Michael M., Liu, Yie
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400118/ https://www.ncbi.nlm.nih.gov/pubmed/36035793 http://dx.doi.org/10.1016/j.xpro.2022.101610

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