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High-throughput microarray reveals the epitranscriptome-wide landscape of m(6)A-modified circRNA in oral squamous cell carcinoma
BACKGROUND: Emerging transcriptome-wide high-throughput screenings reveal the landscape and functions of RNAs, such as circular RNAs (circRNAs), in human cancer. In addition, the post-transcriptional RNA internal modifications, especially N(6)-methyladenosine (m(6)A), greatly enrich the variety of R...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400228/ https://www.ncbi.nlm.nih.gov/pubmed/35999496 http://dx.doi.org/10.1186/s12864-022-08806-z |
Sumario: | BACKGROUND: Emerging transcriptome-wide high-throughput screenings reveal the landscape and functions of RNAs, such as circular RNAs (circRNAs), in human cancer. In addition, the post-transcriptional RNA internal modifications, especially N(6)-methyladenosine (m(6)A), greatly enrich the variety of RNAs metabolism. However, the m(6)A modification on circRNAs has yet to be addressed. RESULTS: Here, we report an epitranscriptome-wide mapping of m(6)A-modified circRNAs (m(6)A-circRNA) in oral squamous cell carcinoma (OSCC). Utilizing the data of m(6)A methylated RNA immunoprecipitation sequencing (MeRIP-seq) and m(6)A-circRNAs microarray, we found that m(6)A-circRNAs exhibited particular modification styles in OSCC, which was independent of m(6)A-mRNA. Besides, m(6)A modification on circRNAs frequently occurred on the long exons in the front part of the coding sequence (CDS), which was distinct from m(6)A-mRNA that in 3’-UTR or stop codon. CONCLUSION: In conclusion, our work preliminarily demonstrates the traits of m(6)A-circRNAs, which may bring enlighten for the roles of m(6)A-circRNAs in OSCC. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12864-022-08806-z. |
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