Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates

AIM: Direct analytical comparison of two major drug-linkers in the antibody-drug conjugate (ADC) field was conducted. METHODS: Four different analytical methods [AlogP calculation, reverse phase (RP) high-performance liquid chromatography (HPLC; RP-HPLC), size exclusion chromatography HPLC (SEC-HPLC...

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Detalles Bibliográficos
Autores principales: Fujii, Tomohiro, Reiling, Calliste, Quinn, Colette, Kliman, Michal, Mendelsohn, Brian A., Matsuda, Yutaka
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Open Exploration 2021
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400747/
https://www.ncbi.nlm.nih.gov/pubmed/36046112
http://dx.doi.org/10.37349/etat.2021.00064
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author Fujii, Tomohiro
Reiling, Calliste
Quinn, Colette
Kliman, Michal
Mendelsohn, Brian A.
Matsuda, Yutaka
author_facet Fujii, Tomohiro
Reiling, Calliste
Quinn, Colette
Kliman, Michal
Mendelsohn, Brian A.
Matsuda, Yutaka
author_sort Fujii, Tomohiro
collection PubMed
description AIM: Direct analytical comparison of two major drug-linkers in the antibody-drug conjugate (ADC) field was conducted. METHODS: Four different analytical methods [AlogP calculation, reverse phase (RP) high-performance liquid chromatography (HPLC; RP-HPLC), size exclusion chromatography HPLC (SEC-HPLC), and differential scanning calorimetry (DSC)] were tested for this comparison. RESULTS: Maytansinoid-based ADCs showed less hydrophobicity than auristatin-based ADCs. Regardless of the drug-linker and drug-to-antibody ratios (DARs), the stability detected by DSC was decreased by conjugation. CONCLUSIONS: The cost and time-efficient analytical comparison described in this manuscript may be useful information for an initial characterization of ADCs prior to detailed biological studies.
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spelling pubmed-94007472022-08-30 Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates Fujii, Tomohiro Reiling, Calliste Quinn, Colette Kliman, Michal Mendelsohn, Brian A. Matsuda, Yutaka Explor Target Antitumor Ther Original Article AIM: Direct analytical comparison of two major drug-linkers in the antibody-drug conjugate (ADC) field was conducted. METHODS: Four different analytical methods [AlogP calculation, reverse phase (RP) high-performance liquid chromatography (HPLC; RP-HPLC), size exclusion chromatography HPLC (SEC-HPLC), and differential scanning calorimetry (DSC)] were tested for this comparison. RESULTS: Maytansinoid-based ADCs showed less hydrophobicity than auristatin-based ADCs. Regardless of the drug-linker and drug-to-antibody ratios (DARs), the stability detected by DSC was decreased by conjugation. CONCLUSIONS: The cost and time-efficient analytical comparison described in this manuscript may be useful information for an initial characterization of ADCs prior to detailed biological studies. Open Exploration 2021 2021-12-31 /pmc/articles/PMC9400747/ /pubmed/36046112 http://dx.doi.org/10.37349/etat.2021.00064 Text en © The Author(s) 2021. https://creativecommons.org/licenses/by/4.0/This is an Open Access article licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, sharing, adaptation, distribution and reproduction in any medium or format, for any purpose, even commercially, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Original Article
Fujii, Tomohiro
Reiling, Calliste
Quinn, Colette
Kliman, Michal
Mendelsohn, Brian A.
Matsuda, Yutaka
Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates
title Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates
title_full Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates
title_fullStr Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates
title_full_unstemmed Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates
title_short Physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates
title_sort physical characteristics comparison between maytansinoid-based and auristatin-based antibody-drug conjugates
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9400747/
https://www.ncbi.nlm.nih.gov/pubmed/36046112
http://dx.doi.org/10.37349/etat.2021.00064
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