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Genetic evidence that uptake of the fluorescent analog 2NBDG occurs independently of known glucose transporters

The fluorescent derivative of glucose, 2-Deoxy-2-[(7-nitro-2,1,3-benzoxadiazol-4-yl)-amino]-D-glucose (2NBDG), is a widely used surrogate reagent to visualize glucose uptake in live cells at single cell resolution. Using CRISPR-Cas9 gene editing in 5TGM1 myeloma cells, we demonstrate that ablation o...

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Detalles Bibliográficos
Autores principales: D’Souza, Lucas J., Wright, Stephen H., Bhattacharya, Deepta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9401136/
https://www.ncbi.nlm.nih.gov/pubmed/36001583
http://dx.doi.org/10.1371/journal.pone.0261801