In silico and in vitro analysis of a rational mutation in gIII signal peptide and its effects on periplasmic expression of rhGH in E. coli

The secretion efficiency of a heterologous protein in E. coli is mainly dictated by the N-terminal signal peptide fused to the desired protein. In this study, we aimed to select and introduce mutations into the – 1, – 2 and – 3 positions of the gIII signal peptide (originated from filamentous phage...

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Detalles Bibliográficos
Autores principales: Ghasemi, Fahimeh, Zare, Hamed, Zomorodipour, Alireza, Shirzeyli, Maryam Hosseinzade, Kieliszek, Marek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Original Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9402515/
https://www.ncbi.nlm.nih.gov/pubmed/36001178
http://dx.doi.org/10.1007/s00203-022-03193-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9402515/
https://www.ncbi.nlm.nih.gov/pubmed/36001178
http://dx.doi.org/10.1007/s00203-022-03193-1

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