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Generation of transgene-free human induced pluripotent stem cells from erythroblasts in feeder-free conditions
This protocol describes the generation and characterization of human induced pluripotent stem cells (hiPSCs) from erythroblasts. A key difference with classical protocols is the reprogramming of erythroblasts from a simple blood draw as opposed to fibroblasts/keratinocytes, which requires a biopsy....
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403557/ https://www.ncbi.nlm.nih.gov/pubmed/36035798 http://dx.doi.org/10.1016/j.xpro.2022.101620 |