Generation of transgene-free human induced pluripotent stem cells from erythroblasts in feeder-free conditions

This protocol describes the generation and characterization of human induced pluripotent stem cells (hiPSCs) from erythroblasts. A key difference with classical protocols is the reprogramming of erythroblasts from a simple blood draw as opposed to fibroblasts/keratinocytes, which requires a biopsy....

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Detalles Bibliográficos
Autores principales: Perriot, Sylvain, Canales, Mathieu, Mathias, Amandine, Du Pasquier, Renaud
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9403557/
https://www.ncbi.nlm.nih.gov/pubmed/36035798
http://dx.doi.org/10.1016/j.xpro.2022.101620

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